Using WCX magnetic solid-phase extraction agent and AUTO M32 fully automatic extractor, extract and purify catecholamine class standards in plasma. Through LC-MS/MS analysis and testing, the linearity is good, the results are accurate and precise, and meet the methodological requirements of basic quantitative testing. At the same time, the magnetic solid-phase extraction technology (mSPE) combined with the fully automated extraction system AUJTO M32 significantly reduces the traditional SPE's pipetting operation and real-time human monitoring, saving labor costs and significantly reducing the impact of human factors in pre-processing.

Therapeutic drug concentration monitoring (TDM) is a common clinical testing item and one of the most widely used applications of LC-MS/MS in clinical testing. The LC-MS/MS detection scheme developed during the development and clinical trial stages of the contract, as well as the accumulated clinical experience, directly guided the implementation of TDM work. At the same time, with the gradual maturity of precision medicine and personalized medication, new requirements have been put forward for the development of TDM.

Angiotensin! Ang I is one of the common clinical biomarkers, and through the Han test of angiotensin extraction efficiency, the clinical applicability and technical performance of various sample pretreatment methods can be effectively evaluated. This article visually compares the performance of a well-known imported brand HLB 96 SPE porous plate and HLB magnetic SPE extractant in extracting Ang I by comparing their methodological indicators. It also provides valuable reference for the development prospects of clinical SPE sample pretreatment technology.