There are many methods for cultivating anaerobic bacteria:

• Physical methodsIncluding air blocking method, boiling method, vacuum method, air displacement method, gas injection method or rotary tube method, etc;

• chemical methodIncluding methods such as pyrogallic acid, wire wool, and sodium hydrosulfite;

• Biological methodsIncluding symbiotic aerobic methods with aerobic bacteria;

• Mixed methodIncluding anaerobic tank (bag) method, anaerobic glove box method or anaerobic chamber method, air displacement wire wool method, air displacement palladium particle method, etc.

Below, we will provide a detailed introduction to several commonly used methods.

Advantages:

1. Relatively easy to operateWithout the need for complex equipment, simply place the prepared materials into the anaerobic tank;

2. Suitable for small and medium-sized experimental needs.

Disadvantages:

1. Gas pak methodThe gas generation bag is disposableAfter use, it needs to be replaced,Long term use results in higher costs；

2. The catalytic effect of the catalyst will decrease after a certain number of usesIt also needs to be replaced regularly;

3. If there is negligence during the operation process(For example)The gas generation bag was not placed in the anaerobic tank in a timely manner after being cut open, and the tank lid was not quickly closed after adding water）Both may lead to the failure of anaerobic environment creation.

Advantages:Low cost and relatively simple operationNo need for complex equipment,Widely used in early anaerobic bacterial culture research.

Disadvantages:

During the reaction processPossible impurities such as NO that may be generatedNot conducive to the growth of some bacteria;

2. While absorbing oxygen,It also absorbs carbon dioxide, which is essential for the growth of certain anaerobic bacteriaThis may lead to the inability of certain anaerobic bacteria to grow well;

3. The control requirements for experimental conditions such as temperature and humidity in the reaction are relatively highIf the conditions are not properly controlled, it will affect the deoxygenation effect;

4. The indicator is not sensitive enoughIt is difficult to accurately determine whether the anaerobic environment meets the requirements.

Advantages:

1. Easy to operate, no need for complex equipment,Simple combination of "airbag production+flexible sealing space"；

2. Rapid deoxygenationUsually:30-60 minutesInternal oxygen can be absorbed, which is beneficial for anaerobic bacteria to quickly enter a growth state.

Disadvantages:

1. Airbags are disposable consumables with high single use costs,For large-scale anaerobic cultivation needs, the amount used is large and the cost will significantly increase；

2. High sealing requirements, if the anaerobic tank cover is not tightened or the sealing ring is aged,Easy oxygen leakage leads to culture failure；

3. Unable to adjust gas composition in real-time,If anaerobic bacteria have special requirements for carbon dioxide concentration, it is difficult to accurately adapt.

The anaerobic glove box method isBy filling the sealed box with a mixed gas and continuously catalyzing the reaction of hydrogen and oxygen to generate water using palladium catalystSo as to actively and continuously maintain a stable anaerobic environment inside the box. Mainly throughThe following three parts work together：

1. Circulating deoxygenation system (core)

System into the boxIntroduce mixed gas(Including N ₂, H ₂, CO ₂ in proportion)H ₂ reacts with residual O ₂ in the gas inside the box on the surface of the palladium catalyst to generate H₂O， Thus consuming oxygen.

The process of deoxygenation in this cycle willongoing，Ensure that any trace oxygen infiltration through the gaps of the transfer chamber or glove operation is immediately removed.

2. Gas displacement chamber (transfer chamber)

This is the entry and exit box for itemsAir lockIt is a sealed small compartment with two doors inside and outside.

Process of inserting items:

1. Open the outer door, put in items, and close the outer door.

2. Start the 'replacement' programInside the cabin, there will beMultiple vacuuming → cycle of filling mixed gasTo eliminate all air (oxygen) inside the cabin.

3. When the gas inside the cabin is consistent with the gas inside the boxOnly then can the inner door be opened and items be moved into the main working box.

3. Sealed box and glove interface

Personnel operate through sealed gloves fixed on the box, while isolating the samples and experiments inside the box from the outside air.

Advantages:

1. Can provide very stable anaerobic conditionsThe oxygen concentration can be reduced to extremely low levels;

2. Multiple experimental operations can be conducted inside the box,Research on solid-liquid cultivation methods suitable for large-scale cultivation of anaerobic bacteria；

3. No need to frequently open the box door, effectively avoiding interference and pollution from the outside air;

4. Some glove boxes have built-in incubators/temperature control systemsIt can achieve precise temperature control, facilitating long-term cultivation and real-time observation.

Disadvantages:

1.The equipment is expensiveMaintenance and upkeep also require high costs;

2. Long term operation of the equipment,High gas consumption and operating costs；

3. Large space occupationRequires a dedicated venue for placement

4. The operation steps are complexIt is necessary to observe the operation of the equipment anytime and anywhere, otherwise operational errors may occur, affecting the experimental results.

Advantages:

1. High gas displacement efficiency, capable offastReduce the oxygen concentration to a lower level;

2. Adjustable gas composition ratioAdapt to the needs of different anaerobic bacteria;

3. No chemical reagent reaction products,Avoid additional inhibition of bacterial growth.

Disadvantages:

1. It is necessary to equip complete sets of equipment such as vacuum pumps, high-pressure gas cylinders, and pressure reducing valves separately,High initial investment cost and occupation of experimental space；

2. The entire process relies on manual operation,The pressure and time of gas extraction/injection are all controlled by experienceThe accuracy of gas ratio is poor, which can easily lead to fluctuations in cultivation results due to operational differences;

3. Lack of real-time monitoring function,Unable to visually determine whether the oxygen concentration and gas ratio in the tank meet the standardsIt requires additional verification through biological indicators, which is time-consuming and cumbersome.

HD-AN series intelligent anaerobic/microaerophilic cultivation system

HD-AN series intelligent anaerobic microaerophilic systemEquipped with high-precision sensors and automatic oxygen control system, it can meet the needs of different bacterial strains,Pre set core cultivation modes such as anaerobic, microaerophilic, and curved bacteria.

1. One-click generationNo need for manual intervention;

2. Quickly generate cultivation environment, significantly reducing waiting time:

• The fastest to reachMicro aerobic conditions<70 seconds

• The fastest to reachAnaerobic environment ＜ 150 seconds

1. The device can be used as needed without any other consumables;

2. Extremely low gas consumption:

• reach, achieve, attainAnaerobic environment gas consumption ≤ 7L/12 petri dish

• reach, achieve, attainMicro aerobic environment gas consumption ≤ 2L/12 petri dish

3. There are abundant options for cultivation tanksTo achieve comprehensive adaptation to various culture carriers——Provide 8 types of culture tanks to choose from：

• Suitable models for placing 6, 12, 24, 36, and 60 conventional culture dishes

• Adaptation tank for specialized double well culture dishes for curved bacteria

• A culture tank that can hold specialized microbial identification carriers such as enzyme-linked immunosorbent assay (ELISA) plates and 96 well plates

• Shigella specific culture tank capable of holding 10 culture bags

System ConfigurationWireless oxygen concentration real-time monitoring systemUsed for the cultivation of anaerobic and microaerophilic microorganisms, cells, etcReal time monitoring of oxygen concentration, carbon dioxide concentration, temperature, humidity, etcEnsure that the cultivation environment meets the experimental requirements.

Machine automatic alarm reminder for exceeding concentration rangeTo help experimental personnel detect changes in equipment status in a timely manner, avoid cultivation risks caused by operational errors or failure to handle them in a timely manner, and ensure the entire cultivation process is guaranteed.

HD-AO series wireless oxygen concentration real-time monitoring system