One 'instrument' can handle multi bacterial cultivation!

Huaduan Biological Anaerobic Microaerophilic Cultivation System Submits Hardcore Transcript

Breaking down cultivation barriers: hardcore practice of multi bacterial cultivation

Campylobacter jejuni: precise adaptation under microaerophilic conditions

Campylobacter jejuni（Campylobacter jejuni）As a typical exampleMicroaerophilic bacteriaIt is an important pathogen that causes acute enteritis in humans,The optimal gas environment for initial separation and cultivation is 5% O₂、10%CO₂And 85% N₂Excessive or insufficient oxygen concentration can affect the normal formation of bacterial colonies. Traditional gas bag cultivation methods often have a wide range of oxygen concentrations and cannot guarantee strict microaerophilic cultivation conditions.

This cultivation system is equipped with an intelligent gas mixing module, which can automatically mix gas concentrations according to preset parameters. In the cultivation experiment,The system accurately controls the oxygen concentration at 5% ± 0.1% and maintains the carbon dioxide concentration at 10% ± 0.1% without any fluctuations throughout the process.

After 48 hours of cultivation, there appeared on the plateMicro convex, semi transparent, moist, non hemolytic dense small bacterial colonies——This is precisely the characteristic manifestation of Campylobacter jejuni.

Neisseria meningitidis: CO2 dependent culture

Neisseria meningitidis（Neisseria meningitidis）It is a pathogenic bacterium that causes epidemic cerebrospinal meningitis,The optimal cultivation temperature is 35-37 ℃, which should contain 5% -10% CO ₂Due to improper timing of specimen collection, high requirements for identification techniques, and easy autolysis of bacteria after isolation, Neisseria meningitidis is rarely isolated in clinical laboratories.

In this cultivation experiment, the system is Neisseria meningitidisAccurately set a carbon dioxide concentration of 5% and quickly generate a cultivation environment, effectively avoiding bacterial death and autolysisAfter 24 hours of cultivation, it was isolated on the plateSmooth, moist, semi transparent non hemolytic, round, gray blue colonies——This is consistent with the standard colony morphology of Neisseria meningitidis.

Streptococcus pneumoniae: flexible cultivation of facultative anaerobic bacteria

Streptococcus pneumoniae（Streptococcus pneumoniae）It is the main pathogenic bacterium that causes community-acquired pneumonia, meningitis, otitis media and other diseases,As a facultative anaerobic bacterium, it grows better and has a more typical colony morphology in a 5% CO ₂ environment.

In this example, the experimenter only needs to preset the facultative anaerobic mode on the operating interface, and the system will automatically adjust the oxygen concentration while introducing 5% carbon dioxide to create a suitable growth environment for Streptococcus pneumoniae.Without manual intervention, the system can maintain parameter stability throughout the entire process, achieving "one click setting and precise control".

In the early stage of cultivation, there appeared on the plateGray and flat colonies, with a moist and semi transparent surface, accompanied by grass green hemolytic ringsAfter 24 hours of cultivation, the center of most colonies will gradually become concave, forming a shape similar to the navel pit. In addition,Some Streptococcus pneumoniae strains can form sticky colonies resembling oil droplets.This cultivation not only demonstrated the system's adaptability to facultative anaerobic strains, but also demonstrated its advantages of convenient, flexible, and efficient operation, saving equipment costs and operating time for clinical laboratories.

Burkholderia cepacia: Cultivation guarantee for aerobic bacteria in Acorus calamus

Burkholderia monocytogenes from Acorus calamus（Burkholderia gladioli）It is an aerobic, motile, non fermenting Gram negative bacterium widely present in natural environments (soil, water sources, etc.) and food (fermented rice flour, Tremella fuciformis, etc.),High concentrations of high-temperature resistant virogens, such as rice yeast acid, are easily produced in facultative anaerobic environmentsFood poisoning incidents caused by this metabolite have occurred in China from time to time, making it a widely concerned bacterium in the food industry.

whichAlthough it can grow in a conventional incubator, fluctuations in environmental parameters may affect its growth status and experimental consistency. In this example, the HD-AN series intelligent anaerobic microaerophilic cultivation system is used toProvide the gas environment required for bacterial growth, achieved the cultivation conditions（28 ℃ aerobic cultivation）Maintaining stability,Provided an ideal and consistent growth environment for bacterial strainsEffectively improved the reproducibility and reliability of the experiment results.

From the final results of plate culture, it can be seen that after 48 hours of cultivation, Burkholderia monocytogenes in Acorus calamus,The surface is smooth and moist, forming a milky white turbid ring. Under oblique light, colonies and the surrounding culture medium surface can be seen with iridescenceIt conforms to the typical morphological characteristics of Acorus tatarinowii.

Product highlight: Technical support behind "multifunctionality"

Scenario Extension: From Clinical Diagnosis to Public Health

Multi directional empowerment

Clinical field: providing "precise pathogens" for disease diagnosis

In clinical diagnosis and treatment,Helicobacter pylori, Bacteroides fragilis, Campylobacter jejuniCultivation of anaerobic and microaerophilic bacteria，Often difficult to achieve due to high environmental requirementsImprove positivity rate, reduce missed detections, and shorten cyclesThe demand.

HD-AN series intelligent anaerobic/microaerophilic cultivation system, throughStable cultivation environment regulation functionAddressing the pain points of traditional cultivation, it can alsoEfficiently process samples and shorten detection time.Currently, over a hundred medical institutions across the country are using it to overcome the difficulty of detecting difficult to cultivate bacteria.

Production application field:Building a 'monitoring barrier' for quality and safety

Food and drug manufacturing enterprisesResearch and development, production, and quality inspection processesAnaerobic bacteria testing is commonly used, such asLactic acid bacteria, Campylobacter jejuni, Clostridium botulinumWait.

The HD-AN series intelligent anaerobic/microaerophilic cultivation system can provide the necessary services for enterprisesEfficient, low-cost, and multifunctionalThe solution，Assist enterprises in efficiently completing research and development, quality inspection, reducing costs, and ensuring product safety and compliance.

Research Exploration Field: Building a 'Stable Platform' for Microbial Research

In the past, due to the lack of cultivation technology for anaerobic microaerophilic bacteria, research on such bacteria in universities lagged far behind aerobic bacteria.HD-AN series intelligent anaerobic/microaerophilic cultivation systemWe are providing more flexible technical support for scientific research, helping to break through the limitations of traditional training.

whether it isResearch on gut microbiota and probiotics, or exploration of fermentation industrial strains and biodegradationBy flexibly regulating the environment,Assist researchers in breaking throughAnaerobic bacterial pure cultivationIdentify technological bottlenecks and explore more research directions.

Public Health Sector: Building the 'First Line of Defense' for Disease Prevention and Control

In the work of the Centers for Disease Control and Prevention and public health institutions,Monitoring, tracing and emergency response of pathogenic bacteriaIt is an important link in ensuring public health safety.This system can efficiently cultivate multiple target strains, providing strong support for rapid response to public health events.

In foodborne disease outbreaks, disease control personnel need toFrom suspicious food and patient samplesRapid isolation and cultivation of pathogenic bacteriaThe system'sAbility to quickly establish an environment and efficient cultivation efficiencyIt can help disease control personnel identify the pathogen in the shortest possible time,Assist disease control personnel in conducting strain typing and epidemic trend analysis.