Old standard:Examination of Campylobacter jejuni.

New standards:expand toCampylobacter jejuni and Campylobacter coliInspection meets more complex and comprehensive food safety inspection needs, with a wider range of application scenarios.

(1) Sample pre-treatment

Old standard:Some samples (general samples, shellfish, egg yolk liquid or egg slurry) are directly homogenized/mixed in selective enrichment solution (Bolton broth), with two-step enrichment at 36 ℃ and 42 ℃.

New standards:Use 0.1% peptone waterRinse, homogenize, centrifuge and enrichTake the sediment suspension and add it to the bacterial growth solution,Cultivate at 42 ℃ for 24 hours under microaerophilic conditions for one-step bacterial growth.

(2) Separation and Purification

Old standard:After bacterial growthDirect line vaccination(Inoculate the 24-hour and 48h cultures and 1:50 dilution separately onto Skirrow blood agar and mCCDA agar plates).

New standards:After adding bacteria, useMembrane culture methodSeparate and utilize the special mobility of curved bacteria to effectively reduce interference from miscellaneous bacteria and improve the detection rate of target bacteria.

Old standard:Bolton broth, modified CCD agar (mCCDA), Skirrow blood agar, Brucella broth.

New standards:Delete the above content and replace with Campylobacter enrichment solution and Kamali blood agar.And the formula of the culture medium was optimized,Better inhibition of bacterial growthMore emphasis is placed on adding to the culture mediumResuscitation enhancing ingredients (such as blood, activated carbon, etc.)Reduce bacterial death caused by oxidative stress and other factors during the inspection process, and improve the detection rate.

(1) Identification of the genus Campylobacter

Old standard (5 tests):Identification will be carried out by combining morphological observation, dynamic observation, oxidase test, microaerophilic growth test at 25 ℃± 1 ℃, and aerobic growth test at 42 ℃± 1 ℃;

New standard (3 trials):Delete power observationCombined withMorphological observation, oxidase test, aerobic 25 ℃± 1 ℃ growth testConduct identification.

Faced with the changes and challenges brought by the new standards, we provideFrom sample pretreatment to final strain identificationofOne stop instrument solutionEnsure a smooth transition in your laboratory and handle it with ease.

HD-DT series automatic sample dilution instrument

As a crucial step in the detection process, this instrument uses a precision balance to weigh the sample that needs to be diluted,Automatically calculate the required diluent based on the weighing results and the set dilution ratioAnd control the precision pump to install diluent, which is easy to operate and lays a solid foundation for the uniformity and reliability of the subsequent bacterial growth process.

HD-H15 Beating Homogenizer

This instrument can efficiently and uniformly prepare sample homogenates through high-speed tapping;Homogenization force(Strike spacing 0-14mm)time(Timer function)speed(1-15 hits per second) adjustableTo adapt to different sample characteristics and fully separate themCampylobacter jejuni/Campylobacter coli contained within and on the surface of "solid and semi-solid" samplesThis lays the foundation for subsequent membrane culture and separation identification.

HD-AN series intelligent anaerobic/microaerophilic cultivation system

Campylobacter jejuni and Campylobacter coli are microaerophilic bacteria that are highly sensitive to oxygen. This system utilizesVacuum pumping and displacementPrinciple, able to quickly and accurately establish and maintain in the culture tankOxygen concentration 5%ofMicroaerophilic environmentSignificantly improve the detection rate.

There are multiple models of matching culture tanks to choose from, supports placing petri dishesBentobacter double well culture dishCone flasks, enzyme-linked immunosorbent assay plates, 96 well plates, etc., can be used offline,Support the full process of microaerophilic protection from inoculation to cultivation, and then to sample transportationEnsure bacterial activity.

System ConfigurationWireless oxygen concentration real-time monitoring system, used for cultivationReal time monitoring of oxygen concentration, carbon dioxide concentration, temperature, humidity, etcEnsure that the cultivation environment meets the experimental requirements.

Machine automatic alarm reminder for exceeding concentration rangeTo help experimental personnel detect changes in equipment status in a timely manner, avoid cultivation risks caused by operational errors or failure to handle them in a timely manner, and ensure the entire cultivation process is guaranteed.

HD-MA series automatic microbial biochemical identification and drug sensitivity system

This system adopts a new type of biochemical phenotype feature interpretation and database comparison to enhance traditional biochemical identification. Based on the national standard requirements for biochemical reactions, the systemCan automatically complete key biochemical test interpretation, equipped with dedicated reagent platesNo need to manually add reagents, accurate and reliable resultsIt conforms to the testing procedures for Campylobacter jejuni and Campylobacter coli, and is an ideal platform for efficient and standardized identification.

HD-M-Discover series microbial mass spectrometry rapid identification and analysis system

This system analyzes the unique protein fingerprint of microorganisms,Obtain rapid and accurate identification results of Campylobacter jejuni and Campylobacter coliIn line with the requirements of the new national standard, it helps laboratories achieve a leap in identification efficiency from "hourly level" to "minute level".

Compared with traditional biochemical identification, the MALDI-TOF MS identification technology recommended by the new national standard,Not only fast, accurate, and wide-ranging, but also capable of achievingPrincipal Component Analysis, Cluster Analysis, Homology Analysis, Bacterial TypingWaiting for mixed bacterial identification functionProvide powerful data support for subsequent traceability analysis.