This study addresses the issue of multiple veterinary drug residues in animal derived foods using ultra-high performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）Technology has established a fast and accurate method for detecting multiple residues. This method has high sensitivity and selectivity, and has been successfully applied to actual sample detection, providing a powerful tool for food safety supervision.

based onFaVEx-NMPurification column and ultra-high performance liquid chromatographyseries connectionmass spectrometry（UPLC-MS/MS）Technology has been developed to simultaneously detect substances in animal derived foods102Residues of veterinary drugsRapid screening and testing methodsBy optimizing the extraction solvent and purification method, the extraction efficiency of the target compound has been improved. At the same time, the chromatographic and mass spectrometry conditions were evaluatedoptimizeenhanceimproverefineThis ensures the sensitivity and accuracy of the method. The detection limit and quantification limit of this method have respectively reached0.04~2.2 μg/kg70 and 0.09~4.4 μg/kgThe recovery rate remains stable at%~120%Relative standard deviation1.34 （ RSD）In%~10.97

%between.The types of veterinary drugs tested in this article include13Sulfonamides102β -Xingxing stimulants, quinolones, macrolides, tetracyclines, nitroimidazoles, glucocorticoids, steroids, tranquilizers, antiviral drugs, chloramphenicol, quinolones and metabolites, fipronil and metabolitescategoryResidues of veterinary drugs. Actual inspectionSample Typeincluding3Pork and beef

and

Germany

Th5 company（2） Sample pretreatment method50 Weigh outgMeat samples₂mL₄In the centrifuge tube, add5g NaSO2Water purifying agent, homogeneous proton, oscillation5 minAdd to include%（V/V）Acetonitrile solution of acetic acid10 mLAs an extraction solution, mix well and shake15 min.6000 r·mincentrifugation5 minTake the supernatant5 mL1passFaVEx-NMPurification column, positive pressure purification, flow rate per second

Drip out, collect all purified liquid, pass through

0.22 μm

Perform machine testing after filtering the membrane.（3） Chromatography and mass spectrometry conditions (omitted here, please feel free to contact and request if needed)（4） The linearity, detection limit, and quantification limit of the method0In order to eliminate the influence of matrix effects on quantitative determination, this experiment used a standard paired curve for quantification. Taking pork as an example, a standard working solution was prepared using blank pork extract. according to0.5“2.3”1.0The method for handling the concentration gradient of standard working solution is as follows:5.0、10.0、20.0、、^-1、The50.0 ng·mLThe matrix spiked solution, according tomass spectrometryTest the conditions and take the concentration of the standard working solution of the spiked matrix as the horizontal axis（x）, vertical axis（y）²To quantify the ion peak area, obtain the standard matrix curve, linear regression equation, and correlation coefficient（R）^{Refer to the national standard}GB/T 33260standard method[121]The detection limit of the calculation method（LOD）And quantitative limit（LOQ

Prednisone

MabofloxacinBenba Ba Bituo（5） Accuracy and precision13To verifyFaVExOne step purification method for determination1Accuracy of veterinary drug residues, validation of spiked recovery rate for negative samples that have been tested.3Three different levels of addition were used（μg/kg5Theμg/kgTheμg/kg）, andmakeuseFaVEx-NM6Purification columnfrontProcessing, parallel measurementSample, repeat three times, calculate the average relative standard deviation（RSD）And the recycling rate. The experimental results are shown in the table12-6，70.16 In μg/kgThe average recovery rate of adding levels is%~112.87%2.96 ， RSDfor3.0%~10.97%70.54 In μg/kgThe average recovery rate of adding levels is%~119%1.34 ， RSDfor5.0%~10.75%69.9 In.27 μg/kgThe average recovery rate of adding levels is%~109%1.73 ， RSDfor

%~10.44

%.