Tea specific columns are key consumables used for sample pretreatment in the field of tea detection. Their core function is to remove interfering substances (such as pigments, tea polyphenols, volatile organic acids, etc.) from tea samples through specific fillers and designs, while enriching target analytes (such as pesticide residues) to improve detection accuracy and sensitivity.
Tea specific columns are mainly used for the detection and purification of pesticide residues in tea. These specialized columns usually use TPT solid-phase materials, which can quickly extract organic acids, catechins, flavonoids and other components in tea, improving detection sensitivity and accuracy.
Packing design
Multi component composite fillers: Through the synergistic effect of different fillers, multi site adsorption of interfering substances in complex matrices is achieved. Ensure effective removal of impurities such as pigments and tea polyphenols, while avoiding dead adsorption of target pesticides.
Selective retention: The filler has a low affinity for the target analyte (such as pesticide residue), ensuring that it is not adsorbed during the purification process, thereby improving the recovery rate.
Column structure
SPE column form: Solid phase extraction (SPE) technology is used to purify the sample through the packing layer inside the column. The material of the column is usually polypropylene, which has good chemical stability and mechanical strength.
Diversity of specifications: Provide products with different packing amounts, column volumes, and specifications according to experimental needs. For example, specifications such as 2g/12mL and 1000mg/6mL are suitable for different sample processing capacities.
The usage method of tea specific columns includes the following steps:
Activation: Add approximately 2 cm of anhydrous sodium sulfate to the tea column, pre wash the column with 5 mL of acetonitrile toluene solution (3:1), and discard the effluent.
Sample loading and elution: Pass 1 mL of the solution to be purified through the column and collect the effluent; Then add 25 mL of acetonitrile toluene solution (3:1) for elution and collect the effluent.
Dissolve again: The eluent is blown dry with nitrogen at 45 ℃, and then dissolved in 1 mL of 60% acetonitrile aqueous solution. It is filtered through a 0.22 μ m nylon filter membrane for machine testing.
These specialized columns are widely used in fields such as tea quality and safety monitoring to ensure that pesticide residues in tea meet relevant standards.