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E-mail
info@psi.bio
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Phone
15873039282
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Address
Chutian Science and Technology Industrial Park, Ningxiang Economic and Technological Development Zone, Changsha City, Hunan Province, China
Chutianpai Biotechnology (Changsha) Co., Ltd
Common troubleshooting and maintenance suggestions for high-throughput peptide synthesizers
Date: 2025-11-21Read: 0
High throughput peptide synthesizerWith the ability of parallel synthesis, the efficiency of peptide library construction has been significantly improved, but its complex flow path system, multi-channel valve group, and precision control system also bring high maintenance requirements. Timely identification and handling of common faults are key to ensuring experimental continuity and data reliability.
1、 Typical faults and troubleshooting methods
Abnormal reagent delivery (leakage/failure to discharge): often caused by aging pipelines, loose joints, or clogged filters. Check if the PEEK tube is cracked, replace the 0.2 μ m inlet filter, and confirm if the pump pressure is normal (usually 5-15 psi).
The decrease in amino acid coupling efficiency may be due to the failure of activators, insufficient resin swelling, or interruption of nitrogen protection. It is recommended to use freshly prepared HBTU/DIPEA solution and ensure that the reaction column is fully pre swollen for at least 30 minutes.
Inconsistent results between multiple channels: commonly caused by distribution valve contamination or calibration deviation. Perform a "channel balance test" to verify the consistency of flow rates in each well using dye solution, and clean or recalibrate the distribution rotor if necessary.
Software communication interruption or error: Check USB/Ethernet connection, update driver program; Some brands need to regularly clean cache logs to prevent system lag.
2、 Daily maintenance suggestions
After each run, execute the "system wide flushing" program and clean the flow path using a gradient of DMF → DCM → isopropanol;
Check the liquid level and sealing of the waste liquid bottle every week to prevent backflow;
Replace the injection needle seal ring and peristaltic pump tube every month;
Verify the positioning accuracy of the robotic arm every quarter to avoid sample deviation.
3、 Preventive measures
Use high-purity (≥ 99%) amino acids and anhydrous solvents to avoid impurity deposition; Establish a consumables replacement record form; When operating in environments with humidity>60%, it is recommended to install a dry nitrogen purge system to prevent hydrolysis of Fmoc groups.
By standardizing operations and actively maintaining, significant reductions can be achievedHigh throughput peptide synthesizerThe failure rate ensures the repeatability and reliability of high-throughput screening data.