ODSfillerIt is a high-performance liquid chromatography method based on octadecylsilane bonded silica gel（HPLC）Fixed phase fillers have high hydrophobicity, high surface bonding coverage, and excellent chemical stability. They are widely used in biochemistry and drug analysis, and can be used to separate various substances such as drugs, natural products, agricultural chemicals, vitamins, etc. They are also commonly used fillers in quantitative analysis of traditional Chinese medicine preparations.

ODSfillerHaving a high carbon content, generally10%-20%Left and right, this gives it stronger adaptability to various types of biomolecules. Its usepHThe scope is usually2-8In addition,ODSThe filler has a narrow particle size distribution, high mechanical strength, and can obtain high-quality peak shapes with high separation.

Operation points and maintenance:

1Loading technology

Homogenization method: Prepare homogenates using solvents with similar particle density to avoid settling and grading.

Pressure control: loading pressure5-12 MPaEnsure that the column bed is uniform.

Flow rate optimization: The loading flow rate is based on the flow rate used2-3Double, reduce hydraulic shock.

2Mobile phase treatment

Filtration and degassing: The mobile phase needs to be subjected to0.22Mmor0.45MmMembrane filtration, ultrasonic degassing10-20minute.

Rinse sequence: Rinse with methanol first10-20Minutes, then switch to the test mobile phase (if containing buffer salt, re distilled water is needed for transition).

3Cleaning and Preservation

Regular cleaning: use100%Wash with methanol or acetone10More than one column volume.

Stubborn pollution: adopting75%acetone:25%Isopropanol or100%Ethyl acetate.