WOL specializes in designing and decorating animal and plant tissue culture laboratories

1、 Overall layout and functional zoning

1. Washing preparation area

Responsible for cleaning, soaking, drying vessels, as well as pre-treatment and disinfection of animal and plant materials. Water supply and drainage *, good ventilation, independent setting, avoiding crossing with clean areas.

2. Preparation area for culture medium/nutrient solution

Used for weighing, preparing, and packaging culture media, it belongs to a quasi clean area and needs to be dry, dust-free, and splash free. It should be strictly physically separated from the sample cleaning area to prevent the introduction of miscellaneous bacteria.

3. Sterilization and disinfection area

Independently set up high-pressure sterilization pots and dry heat sterilization equipment to undertake sterilization work for utensils, culture media, clothing, etc. High temperature resistance, moisture resistance, independent ventilation are required, and direct connection with sterile operating areas is strictly prohibited.

4. Buffer dressing area

As a barrier at the entrance of the clean area, it is used for personnel changing clothes, shoes, hand disinfection, air shower dust removal, maintaining pressure transition, and blocking external pollutants from directly entering the sterile area.

5. Aseptic operation area

The core functional area is used for sterile operations such as inoculation, passage, and cell dissection. The environmental cleanliness reaches level ten thousand, and the interior of the ultra clean workbench is level one hundred, sealed, positive pressure, and dust-free.

6. Training area

Constant temperature and humidity, controllable lighting, used for the cultivation and domestication of animal and plant materials. Set up a light cultivation rack and a constant temperature incubator according to the requirements, ensuring a stable and vibration free environment.

7. Waste Storage and Treatment Area

Located outside the laboratory, it is independently sealed and relatively negative pressure, equipped with sealed garbage bins and independent exhaust, achieving daily cleaning and preventing secondary pollution.

2、 Cleanliness and pressure difference design

Aseptic operation area: Class 10000 (ISO 7), Class 100 ultra clean bench (ISO 5)

Training area: 100000 level (ISO 8)

Buffer room: located between the clean and non clean areas

The overall system adopts gradient positive pressure, and the airflow direction flows from the high clean area to the low clean area, preventing the backflow of miscellaneous bacteria and effectively reducing the pollution rate.

3、 Environmental control system

Equipped with constant temperature and humidity purification air conditioning to ensure stable temperature, humidity, and air exchange rate;

Personnel flow: Dressing, buffering, sterile operation, cultivation exit, no reverse flow

Material flow line: cleaning, preparation, sterilization, aseptic operation, cultivation

Waste flow line: sealed on-site storage area, transported outside for sterilization treatment

Strictly separate the three lines to minimize cross contamination.

5、 Decoration and safety facilities

Clean PVC or epoxy flooring is selected for the ground, and clean color steel plates are used for the walls, with good air tightness and easy disinfection;

WOL specializes in designing and decorating animal and plant tissue culture laboratories