WOL undertakes the design and decoration of the centralized gas supply system for the cell laboratory. The core principles of the centralized gas supply system design for the cell laboratory include cleanliness, stability, sterility, pollution prevention, safety and controllability, and adaptability to the strict requirements of gas purity and pressure for cell culture, proliferation, differentiation and other experiments.
Design and decoration of centralized gas supply system for cell laboratory constructed by WOL
Functional zoning and cleanliness level planning
-
pressPollution risk gradientDivide functional areas into linear and unidirectional arrangements:
Non clean area (office, changing room, disinfection) Buffer room Clean area (C-level background) Core operation area (B-level background+A-level local) High risk area (positive control, cytotoxic operation, negative pressure isolation)
The core operation area (ultra clean workbench, biosafety cabinet, incubator area) needs to reachB-class cleanlinessLocal operation positions (such as sterile inoculation and cell packaging) are equipped with A-level unidirectional flow hoods, with wind speeds controlled at 0.36-0.54m/s; Maintain negative pressure of -5~-10Pa in high-risk areas to prevent aerosol leakage.
Supporting auxiliary area: Independently set up reagent preparation room, sterilization room, and waste disposal room, connected to the core culture area through transfer windows to avoid cross contamination.
Requirements for building and decoration materials
The walls, floors, and ceilings are constructed usingSeamless and easy to clean materialChoose colored steel plates or epoxy resin coatings for the walls, and use a flat epoxy floor for the floor. The internal and external corners should be treated with circular arcs (radius ≥ 50mm) to prevent dust accumulation and dead corners.
The doors and windows are made of stainless steel or aluminum alloy with good sealing performance, and the observation window is made of double-layer vacuum glass; Cleanroom door and buffer room door installationDouble door interlockProhibit simultaneous opening to prevent airflow short circuit.
All pipelines (water, electricity, gas) are concealed and sealed tightly at the wall penetrations; Avoid using wooden materials in the clean area to prevent the growth of microorganisms.
HVAC and Airflow Organization Design
adoptIndependent HVAC systemThe supply air is filtered through three stages: primary, medium, and high-efficiency. The exhaust air needs to be filtered through a high-efficiency filter (HEPA) before being discharged; High risk areas require two-stage HEPA filtration for exhaust.
The airflow direction is strictly unidirectional: Class A local, Class B background, Class C non clean area, adjacent areas have a pressure difference of ≥ 5Pa, and the clean area maintains a positive pressure of+10~+15Pa relative to the non clean area. Pressure difference monitoring and alarm devices are installed.
Airflow organization isTop send backThe return air outlet is located at the bottom of the wall to avoid blind spots in the airflow; Equipment such as incubators and biosafety cabinets should be adapted to the direction of airflow, and exhaust should not interfere with clean airflow.
Character flow and material control
Personnel flow lineNon clean area one shift (take off outer clothing) two shifts (wear clean clothes) air shower room buffer room core area, air shower time ≥ 15 seconds; Entering the A-level operation area requires wearing sterile clothing, double layered gloves, masks, and shoe covers.
Material flow line: The materials are removed from the core area of the dual door interlocking transmission window through outsourcing UV/VHP disinfection and sterilization; Culture medium, serum, etc. need to be sterilized before transfer, and disposable consumables need to be unpacked before entering; Waste is transported outside through a sealed packaging and sterilization dedicated channel, with a one-way closed loop throughout the entire process.
Cell transport adoptsSealed culture bottleTransfer through the transfer window and prohibit personnel from carrying across regions.
Design and decoration of centralized gas supply system for cell laboratory constructed by WOL
