Q ExACTION Electrostatic Field Orbital Trap High Resolution Mass Spectrometer

Q ExACTION Electrostatic Field Orbital Trap High Resolution Mass SpectrometerWidely used in fields such as biological analysis, drug development, environmental monitoring, and food safety. Mainly used for identification and quantitative analysis of proteomics, metabolomics, and biomarkers. Research on drug metabolism and pharmacokinetics, analysis of pollutants in water and soil samples, compound analysis applied in synthetic chemistry and materials science, etc.

Technical parameters of Thermo Scientific Q Exactive mass spectrometer:

Dynamic range between scans:>5000:1

Quality range: 50 to 8000m/z

Resolution: up to 140000

Quality accuracy: Internal:<1 ppm RMS, External:<3 ppm RMS (under specified conditions)

Resolution: 280000 at m/z 200

Maximum scanning speed: 12Hz

Pole Mass Number Filter

Polarity switching<1 second per complete cycle (one full positive ion mode scan and one full negative ion mode scan, with a resolution set to 35000)

Sensitivity Full Scan MS: Column mounted 500fg S/N 100:1

SIM: Column mounted 30kg S/N 100:1

Multi detection capability improves the analysis efficiency of a single cycle, S-Lens ion source

Q ExACTION Electrostatic Field Orbital Trap High Resolution Mass SpectrometerUsage:

Equipment preparation: Ensure that the mass spectrometer is placed in a stable working environment, connected to a power source and gas (such as nitrogen or helium).

Sample preparation: Dissolve the sample in an appropriate solvent according to the analysis requirements, usually using organic solvents or buffer solutions.

Injection system setup: Connect the sample injector or sampler to the mass spectrometer, and set the injection speed and flow rate.

Chromatography system connection: If using liquid chromatography (LC), ensure that the interface between the LC system and the mass spectrometer is well connected, and set appropriate chromatographic conditions (such as flow rate, column temperature, etc.).

Method settings: Create or select analysis methods in mass spectrometry software, set parameters such as ionization mode (positive/negative ions), resolution, scanning range, etc.

Calibration: Perform quality calibration of the mass spectrometer using standard substances (such as retention standards) to ensure the accuracy of the instrument.

Run analysis: Start the analysis program, monitor the instrument's operating status, and collect real-time data.

Data processing: After the analysis is completed, mass spectrometry software is used for data processing and result analysis, generating mass spectra and quantitative results.

The Q ExactiveTM mass spectrometer has an innovative HR/AM QuanfirmationTM function, which can identify, quantify, and confirm more trace level metabolites, pollutants, peptides, and proteins in complex mixtures in a single analysis. Unlike other technologies, this system can obtain extremely reliable analysis results without affecting the sensitivity, quality resolution, or quantitative reproducibility of MS/MS.

The Q Exactive high-performance desktop quadrupole orbital trap LC-MS/MS system has the following significant innovative design, greatly expanding the functionality of Thermo Scientific Exactive family Orbitrap systems:

The integrated quadrupole mass filter achieves precursor ion selectivity. Prior to Orbitrap HR/AM detection, the MS/MS fragmentation process occurred in the collision induced dissociation (HCD) pool with higher energy;

The new C-Trap ion optical system and HCD collision cell provide fast HCD MS/MS scanning and improve the transfer of low mass ions, thereby enhancing sensitivity and quantitative performance, especially suitable for experiments using isotope labels;

Multi detection improves the efficiency of the overall system work cycle, enabling better compatibility with UHPLC and collecting and storing multiple parent ions before simultaneous detection by Orbitrap.

All of these features make the Q Excitation system an ideal choice for accurate quantitative confirmation, capable of identifying, quantifying, and confirming hundreds or thousands of trace components in complex matrices through a single analysis. In addition, the system also provides time-saving workflows for emerging application areas such as food safety and forensic toxicology. These workflows typically use conventional extraction methods, resulting in extremely complex sample matrices for subsequent LC-MS analysis. Once equipped with the Q Exactive system, laboratories can more reliably identify more proteins, metabolites, and pollutants.