Total amount of aflatoxin immunoaffinity column

Aflatoxin is a secondary metabolite produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. It has strong carcinogenicity and has been classified as a Class I carcinogen by the World Health Organization's cancer research agency. It is also a highly toxic substance that is widely present in various grains, nuts, cottonseeds, and products related to human blood and animal feed. The aflatoxin total immunoaffinity column produced by Zhongke Huiren Company can quickly purify aflatoxin B1, B2, G1, G2 from feed, food, grains, and fruits.

Aspergillus flavus is a naturally occurring substance produced by fungi, which has been proven to be prone to cancer in the human body and is a type of carcinogen. The relevant laws of the US federal government stipulate that the content of Aspergillus flavus in human consumption food and cow feed cannot exceed 20ppb, and the content in human consumption milk cannot exceed 0.5ppb. And the content in other animal feed cannot exceed 300ppb.

Produced by Zhongke Huiren CompanyTotal amount of aflatoxin immunoaffinity column-*It can separate Aspergillus flavus B1, B2, G1, G2 from feed, food, grains, and fruits, and isolate Aspergillus flavus M1 from dairy products.

Scope of application and performance

It can specifically purify Aspergillus flavus B1, B2, G1, G2 in the sample.Total amount of aflatoxin immunoaffinity column-*Widely used in the pretreatment of samples such as grain, food, feed, nuts, etc.

Column capacity: ≥ 300ng

Recovery rate: ≥ 85%

principle

The basis of this experiment is the specific reaction between antigen and antibody. The monoclonal antibody against Aspergillus flavus was coupled to the in column gel, and the Aspergillus flavus in the sample was extracted, filtered and diluted; Then, the sample extract is passed through an affinity column bound with Aspergillus flavus antibodies. At this point, Aspergillus flavus binds to the antibody in the affinity column. Afterwards, use distilled water to remove impurities from the immunoaffinity column. Wash the affinity column with methanol to separate Aspergillus flavus from the antibody. Afterwards, inject the filtered methanol solution into a fluorescence meter or HPLC for measurement.

Usage characteristics:

·Quick - Each sample only takes 25 minutes (including sample pretreatment)

·Sensitivity - detection limit can reach 1 ppb, while HPLC can reach 0.1 ppb

·Wide detection range -0-300ppb

·Convenient - Use the same sample extraction method and instrument to detect other fungi

·Simple - No need for any special extraction or special location

·Safety - No need for standard products to be tested, safe for operators, safe and reliable operation, simple and convenient.

Advantage:

1. Reliable performance, certified by multiple domestic enterprises and institutions;

2. Professional service, years of technical service experience, and participation in the formulation of national and industry standards;

3. Imported quality, domestic brand.