Topological pattern of cell substrate stiffness and hardness

Cell substrate stiffness regulation micro patterns and coupled perfusion and pressure stimulation culture

Features:

Control the 3D structure and mechanics of cells

Cells are cultured in a flat or microstructured soft 3D environment to mimic in vivo conditions.

The stiffness of the substrate can be selected from very soft (1 kPa) to very hard (200 kPa)

Provide various substrate morphologies (flat, circular holes, square holes, grooves, etc.)

The substrate based on gel is ready for your cell culture experiment

Due to the fact that cells are directly seeded on top of features (which makes it easy to restrict non migrating cells), it is easy to use and user-friendly

Pre coated ECM matrix (such as fibronectin)

Suitable for any cell culture substrate (cover glass, culture dish, porous plate)

The optical transparency of gel makes these substrates compatible with high-resolution optical microscope systems

PUBLICATIONS

• Cardiac spheroids as promising in vitro models to study the human heart microenvironment
  Polonchuk, L.,et al. Scientific reports, two thousand and seventeen7(1), 7005
• Current and emerging modalities for detection of cardiotoxicity in cardio-oncology
  Khouri, M. G.,et al. Future cardiology,2015 11(4), 471-484
• Bioinspired living structural color hydrogels
  Fu, F., et al.Science Robotics, 2018 3(16), eaar8580
• Preparation of hydrogel substrates with tunable mechanical properties
  Tse, J. R., & Engler, A. J.Current protocols in cell biology, 2010 47(1) 10-16
• Differentiation of liver progenitor cell line to functional organotypic cultures in 3D nanofibrillar cellulose and hyaluronan-gelatin hydrogels
  Malinen, M. M.,et al.Biomaterials,2014 35(19), 5110-5121.

Products available for ordering:

1Coverslip coated with gel

---Adjust the stiffness of the substrate to reproduce the in vivo environment

A polyacrylamide gel coated cover slide for cell culture is provided.
The range of available substrate stiffness is similar to the in vivo mechanical properties of naturally embedded cells.
As the cover glass is ready, cells can be easily and quickly cultured.
Multiple types of rigidity:
From very soft (1 kPa) to very hard (200 kPa)
Available anytime:
Directly inoculate cells
Optical Transparency:
Easy to observe optically

Coated with fibronectin
Compatible with high-resolution optical microscope systems
Provided in a separate plastic bag in the form of water-based solution to maintain the characteristics of gel
Standard size: 24 mm circular cover glass (approximately 170 μ m thickness)
Compatible with infusion chamber
Specification requirements can be customized;
Coverslip coated with gel Contents:

Cover glass coated with microstructure gel

---Simultaneously controlling shape and stiffness to reproduce the internal environment

A micro structure polyacrylamide gel coated cover slide for cell culture is provided.
The gel based matrix comprises soft or rigid microstructures in the form of open microchannels (grooves) or pores.
Therefore, cells can be cultured on substrates that simulate the terrain features and stiffness of the in vivo environment.
>always available
Directly inoculate cells
>Multiple types of rigidity
From very soft (1 kPa) to very hard (200 kPa)
>Extensive 3D design
Groove, square hole, circular hole, etc

Coated with fibronectin, therefore it can be used

Compatible with high-resolution optical microscope systems

Provided in a separate plastic bag in the form of water-based solution to maintain the characteristics of gel

Provide standard shapes: grooves and circular holes

Standard size: 24 mm circular cover glass (approximately 170 μ m thickness)

Compatible with infusion chamber

Typical arrangement of standard 24mm arc-shaped grooves and grooves:
Each cover slip has eight areas, characterized by varying widths (grooves) or diameters (circular holes): from 10 μ m to 100 μ m.
The depth of the structure is 10 μ m.
The following scheme describes the special case of circular wells, but the distribution is similar in the case of grooves

Additional Information:

The size of the cover glass can be modified as needed

The same type of gel can be adapted to culture dishes or porous plates

Other shapes of triangular holes, square holes, or rectangular holes can be manufactured as needed

Other depths can be manufactured according to requirements

2、 Flexcell substrate stiffness adjustable cell tensile loading culture system from the United States

Stretchable cell substrate hardness control culture dish（CellSoft™ 100mm Round Dishes）

Cells sense soft! CellSoft™ offers softer substrates to match the material properties of tissue niches to better meet the needs of biological laboratories wanting to grow their cells on native stiffness。

●Range of elastic modulus1-80kPa

diameter100mmCulture dish, with a total growth surface area of57cm²

●Optional porous culture plates60mmand100mmPetri dish

●BioFlex®CellSoftstandard6orifice plate

●Pulling cells on a flexible substrate

●Chamber glass slideCellSoft

●Surface protein coating, sterile individual packaging

CellSoftThere are many different types of culture plates, such as different hardness, different well plates, chamber slides (circular porous plates) used for microscopic observation, covalent coatingCollagenIOr other proteins that can provide static or dynamic tensile stress stimulation to cells. More importantly, the new typeCellSoftThe culture plate can be repeatedly digested with trypsin and inoculated with cells, and the protein coated surface can be reused up to three times.

Soft silicone elastomer coated culture dish.
Modulus range: 1-80 kPa
Ideal choice for the expansion of passaged cell lines
● Optically transparent, allowing direct observation of cells through inverted or upright microscopes (membrane thickness: 1000um)
Covalent bonding surfaces: Coated with amino groups, collagen (type I or IV), elastin, ProNectin (RGD), and laminin (YIGSR), as well as untreated.
Low self fluorescence, can be used for immunohistochemical analysis or fluorescent probes.
Store at room temperature in the dark or without direct sunlight for up to 1 year.

Background:

Cells like softnessFlexcell®Founder and CEO Dr. Albert Banes said, "Over the years, cell culture has undergone changes, but the two popular innovations are the ability to grow and stretch cells on flexible growth surfaces and control substrate rigidity. Flexcell's new CellSoft ™ Cultivating the product further drives these innovations, and we know that researchers will love this product

“Cells like soft!” says Flexcell® Founder and President, Dr. Albert Banes, Ph.D. “Cell culture has met with change over the years, but two of the hottest innovations have been the ability to grow and stretch cells on flexible growth surfaces and to control the rigidity of the substrate. Our new CellSoft™ culture ware advances those innovations even further, and we know researchers will appreciate this product.”

Flexcell, a company based in the United States, commercialized the growth surface of elastomers in 1988 and provided a method device for controllably stretching cells based on the mechanics of heart, blood vessels, lungs, or other mechanically active tissues. Compared to glass or polystyrene culture plates, these elastomers have a "soft" and stretchable surface. With CellSoft ™， Flexcell ® Can create a softer matrix to matchOrganize niche (stem cell microenvironment)To better meet the needs of biological laboratories that wish to grow cells on natural rigid substrates based on their material properties.

Notes:The cradle like environment surrounding stem cells protects them, and this environment is calledniche.nicheNot only does it provide nutrients for stem cells, but it also guides their actions and determines the direction of their differentiation.

Flexcell® wasfirst to commercializeelastomer growth surfaces in 1988 and toprovide the meansto controllably stretch cells according to the mechanics of the heart, blood vessels, lungs, or other mechanically active tissues. These elastomer surfaces are“soft” and stretchable compared to glass or polystyrene culture plates. With CellSoft™, Flexcell® has created even softer substrates to match the material properties of tissue niches and better meet the needs of biological laboratories wanting to grow their cells on native stiffness substrates.

Flexcell®It is recommended that users cultivate their reserve cells on a given rigid substrate matrix coating and conduct experiments on the same type of surface. In this way, cells will not experience the "matrix shock" of transferring from one hardness and matrix coating to another hardness.

CellSoft™ Has various stiffness and plate formats (disc and porous plate). It can covalently bond with collagen I or other matrices, and has been pre sterilized and ready for use. CellSoft ™ A 100mm circular culture dish is ideal for passaging cells and other flat forms, including a flexible bottom Bioflex ® Flat plate for conducting static or dynamic tensile experiments.

CellSoftThere are many different types of culture plates, such as different hardness (elastic modulus range 1-80kPa), chamber slides (circular porous plates) that can be used for microscopic observation, covalently coated with Collagen I or other proteins, which can perform static or dynamic tensile stress stimulation on cells. More importantly, the new CellSoft ™ The culture plate can be repeatedly digested with trypsin and inoculated with cells, and the protein coated surface can be reused up to three times

●Range of elastic modulus1-80kPa

●Optional porous plate60mmand100mmculture plate

●BioFlex®CellSoftstandard6orifice plate

●Pulling cells on a flexible substrate

●Chamber glass slideCellSoft

●Surface protein coating, sterile individual packaging

Amino,

Collagen (Type I or IV),

Elastin,

ProNectin (RGD),

and Laminin (YIGSR)
and untreated(Unprocessed)

CellSoftThere are many different types of culture plates, such as different hardness, different well plates, chamber slides (circular porous plates) used for microscopic observation, covalent coatingCollagenIOr other proteins that can provide static or dynamic tensile stress stimulation to cells. More importantly, the new typeCellSoftThe culture plate can be repeatedly digested with trypsin and inoculated with cells, and the protein coated surface can be reused up to three times.

Combined with Flexcell FX-5000 or 6000T cell tension and stretching systems, substrate hardness control tension and stretching can be achieved.

Highlights:
1）The system provides axial and circumferential stress loading for various cultures of two-dimensional, three-dimensional cells and tissues; Not only does it have biaxial tensile force loading, but it also has uniaxial force application function
2）A computer-controlled stress loading system that provides controllable, reproducible, static, or periodic stress changes for cells cultured in vitro.
3）Using a vacuum pump, stretch the elastic silicone mold at the bottom of the culture plate. The high stretchability of the bottom of the cell culture plate can reach 33%, and the stress can be automatically adjusted and controlled through a gas device.
4）Based on flexible membrane substrate deformation and uniform stress distribution;
5）Real time observation of the response of cells and tissues under stress;
6）*The flexstop isolation valve can make a portion of the cells in the same culture plate subjected to force, while a portion of the cells in the same culture well are not subjected to force, facilitating comparative experiments;
7）Integrated with pressure transmission instrument, it also has multi-channel cell pressure loading function;
8）Paired with Flex Flow parallel plate flow chamber, it can apply fluid shear stress while pulling cells;
9）Up to 4 channels, capable of running 4 different programs simultaneously, for conducting multiple comparative experiments on different tensile deformation rates;
10）Multiple frequencies, amplitudes, and waveforms can be run in the same program;
11）There are various types of simulated waveforms available for loading: static waveform, positive rotation waveform, cardiac waveform, triangular waveform, rectangular waveform, and various specially designed waveforms;
12）Better control of waveforms under ultra-low or ultra-high stress;
13)The computer system intelligently regulates the loading cycle, magnitude, frequency, and duration of tensile and tension forces
14)Loading and analyzing the biochemical reactions of various cells under tensile stress stimulation
15)Wide range of extension: 0-33%
16)Wide pulling frequency range: 0.01-5Hz

17）More than 4000 successful literature have been published and used by 150 domestic institutions.

3、 Cell micro pattern tensile and fluid shear stress loading culture system

Flexcell substrate topology micro pattern tension and stretching culture system, topology micro pattern compression and fluid shear stress, cell micro pattern tension system, cell micro pattern compression system, cell micro pattern fluid shear stress system

Model:Flexcell substrate topology micro pattern in the United States
Brand:Flexcell from the United States

Flexcell substrate topology micro pattern tension and stretching culture system in the United States

Flexcell Micro Pattern Compression and Fluid Shear Stress System in the United States

The life activities of cells are regulated by extracellular signaling molecules, which mainly include biochemical signals (hormones, vitamins, etc.) and physical signals (elasticity, fluid shear force, matrix topology, etc.). Exploring how cells perceive and respond to extracellular signaling molecules is one of the focuses of cell biology research. Extracellular topology is a general term for the morphology of the microenvironment in which cells survive, and many tissues in the body contain natural substances ..

(Also provide Flexcell substrate topology micro pattern compression and fluid shear stress cultivation system)

The United States has newly launched flexible substrate membrane stretch culture plates with cell micro patterns (6-well and 24 well), which give the stretch culture plates a biomimetic surface morphology, allowing cells to extend and grow in the direction of the nano patterns.

Nano patterned stretching and compression culture surfaces provide a cellular microenvironment that mimics the aligned structure of natural extracellular matrix, promoting the development of cellular structure and function.

• Biomimetic aligned nanoscale surface morphology
• Nano morphology orientation

Highlight: Micro patterns can be customized according to needs

Highlights:

1）The system provides axial and circumferential stress loading for various cultures of two-dimensional, three-dimensional cells and tissues; Not only does it have biaxial tensile force loading, but it also has uniaxial force application function
2) A computer-controlled stress loading system that provides controllable, reproducible, static, or periodic stress changes for cells cultured in vitro.
3) Using a vacuum pump, stretch the elastic silicone mold at the bottom of the culture plate. The high stretchability of the bottom of the cell culture plate can reach 33%, and the stress can be automatically adjusted and controlled through a gas device.
4) Based on flexible membrane substrate deformation and uniform stress distribution;
5) Real time observation of the response of cells and tissues under stress;
6) The flexstop isolation valve of * can make a portion of the cells in the same culture plate subjected to force, while a portion of the cells in the same culture well are not subjected to force, facilitating comparative experiments;
7）Integrated with pressure transmission instrument, it also has multi-channel cell pressure loading function;
8）Paired with Flex Flow parallel plate flow chamber, it can apply fluid shear stress while pulling cells;
9）Up to 4 channels, capable of running 4 different programs simultaneously, for conducting multiple comparative experiments on different tensile deformation rates;
10) Multiple frequencies, amplitudes, and waveforms can be run in the same program;
11) There are various types of simulated waveforms available for loading: static waveform, positive rotation waveform, cardiac waveform, triangular waveform, rectangular waveform, and various specially designed waveforms;
12) Better control of waveforms under ultra-low or ultra-high stress;
13) The computer system intelligently regulates the loading cycle, magnitude, frequency, and duration of tensile and tension forces

FlexcellTopology micro pattern compression system

The United States has launched a new flexible substrate membrane compression culture plate (6-well) with cell micro patterns, which enablescompressThe culture plate has a biomimetic surface morphology, and cells can extend and grow in the direction of nano patterns.

Nano patterned stretching and compression culture surfaces provide a cellular microenvironment that mimics the aligned structure of natural extracellular matrix, promoting the development of cellular structure and function.

• Biomimetic aligned nanoscale surface morphology
• Nano morphology orientation

highlight

1）The system provides periodic or static pressure loading for various tissues and three-dimensional cell cultures;
2) Based on flexible membrane substrate deformation and uniform stress distribution;
3) Real time observation of the response of cells and tissues under pressure;
4) Selective blocking of stress loading on cells;

5）Simultaneously possessing multi-channel cell tensile loading function;

6）Up to 4 channels, capable of running 4 different programs simultaneously, for conducting multiple pressure deformation rate comparison experiments;

7）Multiple frequencies (0.01-5 Hz), amplitudes, and waveforms can be run in the same program;
8）Better control of waveforms under ultra-low or ultra-high stress;
9）Multiple types of waveforms: static waveform, positive rotation waveform, cardiac waveform, triangular waveform, rectangular waveform, and various specially designed waveforms;
10)Intelligent control of pressure loading cycle, size, frequency, and duration by computer system

FlexcellTopological micro patterned fluid shear stress system

· Micro patterns can be customized.

· Provide various forms of fluid shear stress to cells: steady flow shear stress, pulse shear stress, or reciprocating shear stress.

· After being coated with special matrix proteins25x 75x 1.0mmCultivate cells on cell culture slides.

· up to6Channels, each containing different slides, can cultivate different cells

· The computer-controlled peristaltic pump can adjust the magnitude of shear stress from0-35 dynes/cm²

· throughOsci-FlowThe liquid controller provides reciprocating or pulsed fluid shear stress.

· Detect the arrangement reaction of cells under the action of fluid flow.

· The equipment is easy to disassemble and can be sterilized at high temperatures.

· Can be wrapped in special packaging6Simultaneously culture cells on a single cell culture slide.

· Provide two liquid flow pulse dampers.

Note: Published by Yang Qingchen

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Topological pattern of cell substrate stiffness and hardness