Tissue Culture Room

GuizhouTissue Culture RoomManufacturer's guidance

（1) Design specifications and instructions

1. The construction area is 30m ². The main components include: box insulation structure, insulation door, temperature control equipment (indoor and outdoor units), humidity control equipment, control system, remote monitoring, plant lighting system, sterilization system, fresh air system, and indoor pipeline lines. The requirement is to reach the * level of similar domestic products, simulate and reproduce various meteorological conditions in nature, and accurately control indoor operating parameters such as temperature, humidity, and lighting according to experimental requirements.

（2) Performance parameters

*1. Temperature control range: 16-35 ℃, control accuracy: ± 1 ℃, indoor temperature uniformity ≤ ± 1.5 ℃;

*3. Light source illumination control: Grouping control can be performed for ≥ 160umol/s/㎡ at 10cm below the lamp;

（3) Main configuration indicators

1. Materials of thermal insulation enclosure structure of equipment room: pentahedron structure is adopted, and double-sided sandwich polyurethane color steel thermal insulation board is adopted. The thickness of the board is 50mm, the thickness of the steel plate is ≥ 0.5mm, flame retardant, and the self extinguishing time is less than 5s. The specific gravity of polyurethane foam is 40kg/m3, and the thermal conductivity of foam is ≤ 0.021W/(m2 * K); Water absorption rate ≤ 2.2% (v/v). Fire rating: B1 level (with quality supervision bureau inspection report).

2. The indoor corners are decorated with purified aluminum profiles, and the indoor corners are all curved aluminum decorations, which are beautiful and have no hygiene blind spots.

3. According to the indoor area, equipped with three proof lighting and ultraviolet sterilization lamps.

4. Ground: Anti slip and wear-resistant PVC flooring with a thickness of ≥ 2mm is laid on the ground.

5. Thermal insulationDoor: The insulation door adopts polyurethane sealed insulation door. The indoor joints are well connected and sealed, designed and implemented according to the requirements of the sterile room, with dimensions:W80 * H190cm * ± 2cm polyurethane purification board airtight door with glass observation window, allowing for real-time observation of indoor conditions through the observation window.

6. Cultivation rack: Each room is equipped with a cultivation rack with dimensions of 1250 * 50 * 2000mm;Each group of culture racks is5 layers of trays, including 5 layers with light sources,Each layer configuration5 LED plant growth lights; The height of each layer can be freely adjusted up and down by adjusting the fixing parts on both sides of the cultivation rack. The entire set of cultivation racks is made of stainless steel material and has an assembled structure that can be disassembled and assembled at will.

*7. Plant supplementary light source:

*7.1 Each cultivation rack is equipped with 5 LED plant growth lights on each layer of the light source, with a minimum of 160umol/s/㎡ at 10cm below the lights (adjustable grouping);

*7.2. Power of LED plant growth lamp: 18W, powered by 220V;

*7.3. The spectrum of the LED plant growth lamp light source should include at least three bands: R, G, and B;

*7.4 Spectral structure: 380-780nm continuous spectrum, suitable for plant growth full spectrum plant growth lamp;

*7.5. Light source control: At least group dimming can be carried out according to growth needs, and each group of culture racks can be independently controlled by switches. The light source can be controlled by the system to set the grouping and dimming of the light sources on the cultivation rack, as well as the opening time of the light sources;

8. Airflow circulation structure: The airflow circulation structure supplies air from the side wind wall orifice plate, passes through the cultivation areas on both sides, and returns air from the top of the center to maintain a constant indoor temperature and humidity. A diffuser cover is installed at the inlet of the evaporator to filter and trap unclean air particles in the diffuser cover, preventing them from entering the air duct circulation and improving the cleanliness of the air inside the box. The overall air wall orifice plate has uniform openings and smooth air outlet; The overall wind wall is made of aluminum alloy material and assembled using modular design, which is easy to disassemble and assemble for future maintenance, replacement, and cleaning. The aperture is not less than 5mm.

*9. Temperature control technology: Two sets of vortex temperature control units are used for temperature control. The compressor unit is controlled by PLC, and the required indoor temperature and setting requirements are distinguished through system logic thinking. The number of compressor operations can be freely controlled and switched to meet indoor problems while achieving energy-saving effects and the service life of the compressor. The compressor unit should also have dust-proof and anti floc configurations to prevent high pressure protection caused by full suction of the condenser. The indoor unit adopts a composite cold air unit, which is designed with dual system temperature control and intelligent defrosting control.

*10. Moisture control technology: Refrigeration dehumidification units are used for dehumidification, and drainage issues are properly handled.

11. Fresh air exchange system: using a fully heated fresh air exchange, it can discharge the turbid indoor air while introducing fresh outdoor air into the room. The fresh air volume can reach 400m ³/h;

12. Control system: The control box adopts galvanized steel sheet insulated distribution box,Embedded,There are short-circuit protection, leakage protection, phase sequence protection, missing item protection, overcurrent protection, high and low voltage protection and other devices inside. The touch screen is operated using15 inch color LCD touch screen and equipped with PLC programmable logic controller, with simplified Chinese operation mode; It also has alarm functions such as high and low pressure alarm, high and low temperature alarm, and abnormal flow switch alarm. Display text information on the LCD touch screen interface and have a confirm button to release the alarm. At the same time, the device is also equipped with an independent telephone alarm system. When the temperature of the device exceeds the limit.

13. Main functions of the control system: display current status, set control parameters, control temperature and humidity, temperature and humidity curve chart, lighting control, fresh air control, alarm recording, power on self operation function, temperature and humidity recording. Temperature and humidity records should be ≥ 10000, with a memory capacity of ≥ 8G. Auxiliary functions such as saving data through USB flash drive or storage card, alarm recording function, and data Excel export should be available, and data should be stored for at least 5 years; The control system can set temperature, humidity, and other functions and operating cycles for multiple time periods every day;

14. The real-time video monitoring system can record and store the entire process of indoor biological growth in the climate chamber, which can be accessed and viewed later. At the same time, during the monitoring process, a real-time video image of the scene is automatically captured and linked with the on-site environmental data to form a chain system information database. In the later stage, the system can query and retrieve real-time environmental data, on-site image data, and animal and plant morphology image data of a certain period in history. Daily reports of animal and plant growth data are automatically generated and stored, and the reports are automatically cataloged and archived by year and month. The data reports can be stored for more than 10 years, and permission allocation can be made for data viewing. Only authorized users can view the corresponding reports.

15. Remote control function, which can be connected through Ethernet or WIFI, allows real-time monitoring of indoor temperature, humidity, lighting conditions from anywhere through a computer, tablet, or mobile phone. It can also remotely set the temperature, humidity, lighting intensity, and other parameters that can be set locally in the climate chamber. Free mobile app monitoring software and cloud services are provided.

GuizhouTissue Culture RoomManufacturer's guidance