Th1/Th2/Th17 cytokine detection kit

Beadstar-mPlex®MouseTh1/Th2/Th17 cytokine detection kit

Detection of cytokines: IL-2, IL-4, IL-6, IL-10, TNF - α, IFN - γ, IL-17A

Detecting cytokines:

Can detect 7 cytokines: IL-2, IL-4, IL-6, IL-10, TNF - α, IFN - γ, IL-17A

Detection method: Flow cytometry fluorescence method, easy to operate, saves samples, ordinary flow cytometry model (with APC and PE channels)

Detection method: Flow cytometry fluorescence method

Sample: Cell culture supernatant, serum

Required sample size: serum (5-10 microliters); Cell supernatant (25-50 μ L)

Suitable models: Strong universality, can be detected by ordinary streaming models

Beadstar-mPlex®Th1/Th2 Mouse 5 Cytokine Combined Detection Kit

Detection Principle

This kit is based on flow cytometry analysis, and the detection principle is shown in Figure 1. Corresponding human cytokine antibodies are attached to specific fluorescent encoded microspheres to capture cytokines in the solution. When these cytokines are present in the detection sample, the capture antibodies linked to the fluorescent encoded microspheres will bind to them, and finally form a microsphere linked capture antibody antigen PE labeled detection antibody complex by adding specific detection antibodies labeled with phycoerythrin (PE). Under the flow cytometry phycocyanin (APC) and phycocyanin-Cy7 (APC-Cy7) channels, 12 groups of microspheres were recognized and used to determine antigens (Figure 2); In the PE channel of a flow cytometer, the fluorescence intensity of PE on microspheres is positively correlated with the concentration of each analyte in the sample. The corresponding fluorescence signals of each calibration standard can be fitted into a concentration fluorescence intensity standard curve, and the concentration of each antigen can be calculated through the curve equation.

Figure 1 Schematic diagram of high-throughput detection of cytokines by flow cytometry (taking 6 cytokine detection as an example)

Figure 2. Cytokines corresponding to each microsphere

Our advantages:

professionalThis series of products is developed by a doctoral team with solid research foundation and rich experimental experience in multiple disciplines such as biology, materials, and medicine. Through multiple exploratory tests, the quality is excellent.

quality controlBefore being released, this series of products will undergo strict quality control to ensure their quality.

Technical ServiceWe have professional technical guidance to solve all the problems you encounter in the experiment.

Short delivery cycleShipment within one week.