Th1/Th2/TH17 Mouse Cytokine Combined Detection Kit

Beadstar-mPlex ® Mouse Th1/Th2/Th17 Cytokine Combined Detection Kit

This kit is used for quantitative analysis of mouse culture cell supernatant and mouse serum samplesIL-2TheIL-4TheIL-6TheIL-10TheIL-17ATheIFN-γTheTNF-αThe concentration. This reagent kit is based on flow cytometry analysis, and the detection principle is shown in the diagram1As shown, in7Attach corresponding mouse cytokine antibodies to specific fluorescent encoded microspheres. When specific antigens are detected in the sample, these capture antibodies linked to fluorescent encoded microspheres will bind to them, and finally, phycoerythrin will be added（PE）Labeling specific detection antibodies to form microsphere linked capture antibodies-antigen-PEThe labeled detection antibody complex. Phycocyanin in flow cytometry（APC）Under the channel,7Cluster microspheres are recognized and used to determine antigens; In flow cytometryPEUnder the channel, on the microspherePEThe fluorescence intensity is positively correlated with the concentration of each detected substance in the sample, and the corresponding fluorescence signals of each calibration standard can be fitted into a concentrationThe fluorescence intensity standard curve can be used to calculate the concentration of each antigen through the curve equation.

Required sample size：Serum (5-10 microliters); Cell supernatant (25-50 μ L)

Suitable models:Strong universality, can be detected by ordinary streaming models

Kit composition:（100 T ,Measurable80One sample and two standard curves)，4 °Csave

Fluorescent encoded microspheres and corresponding detection substances

High sensitivity and wide linear range

Beadstar-mPlex ® The reagent kit has excellent sensitivity and can detect 1 pg/mL,linear rangeAt 10-2500 pg/mL, the accuracy and reliability of the test results are ensured. This means that the kit can accurately and reliably detect the content of cytokines, providing accurate data support for researchers.

Our advantages:

professionalThis series of products is developed by a doctoral team with solid research foundation and rich experimental experience in multiple disciplines such as biology, materials, and medicine. Through multiple exploratory tests, the quality is excellent.

quality controlBefore being released, this series of products will undergo strict quality control to ensure their quality.

Technical ServiceWe have professional technical guidance to solve all the problems you encounter in the experiment.

Short delivery cycleShipment within one week.