Tawang Technology Blood Oxygen Binding Test System

Product description

The affinity between hemoglobin and oxygen affects the oxygen content in arterial blood. Hemoglobin oxygen saturation is50%The oxygen partial pressure at that time is calledP50It is a commonly used indicator that reflects the affinity between hemoglobin and oxygen, as well as the oxygen release function of hemoglobin. The oxygen binding capacity testing system is a device used to record the oxygen balance curve (the binding and release process of hemoglobin and oxygen) in the blood.

Based on deoxyhemoglobin (Hb) and oxygenated hemoglobin (HbO)₂）The light absorption characteristics of hemoglobin are different. The oxygen binding capacity testing system uses dual wavelength spectrophotometry to detect the light absorption value of hemoglobin. After calculation formula conversion, it is used as the Y-axis, and the absolute value of oxygen partial pressure (unit mmHg) in the sample is measured using a fluorescence quenched oxygen sensor. As the X-axis, the oxygen binding and dissociation curves are plotted.

The blood oxygen binding capacity testing system can detect dissolved oxygen in blood, red blood cell lysate, hemoglobin products, etc., and obtain the binding and dissociation curves of hemoglobin and oxygen through software analysis functions, automatically analyzing P50 and Hill coefficient.

Product Features

1. Fully automated testing

-Fully automated testing greatly reduces manual operations and avoids errors introduced by human intervention;

-The entire measurement process is temperature controlled;

2. Professional control analysis software

-All settings are automatically executed through software;

-Customizable editing experiment process, the system automatically executes the sequence of editing actions;

-Real time hemoglobin measurement and oxygen partial pressure measurement, automatic analysis;

-One click export of data reports;

3. Analyze data

-Oxygenation curve

-Oxygen dissociation curve

-Oxygen saturation

-Oxygen partial pressure

- P50

-Hill coefficient

Measuring principle

The blood oxygen binding capacity testing system is based on the different light absorption characteristics of deoxyhemoglobin (Hb) and oxygenated hemoglobin (HbO2), and uses dual wavelength spectrophotometry to detect the light absorption value of hemoglobin. The change in absorption value reflects blood oxygen saturation. Measure the absolute value of oxygen partial pressure in the sample using a fluorescence quenching oxygen sensor, and the software automatically plots the oxygen binding and dissociation curves.

Fluorescence quenching oxygen partial pressure measurement

The oxygen partial pressure measurement of the blood oxygen binding force testing system is based on the fluorescence quenching technology of *, the principle of oxygen attenuation of fluorescent substances, and the use of micro fiber optic sensors to monitor the changes in oxygen partial pressure in the sample solution. The response speed is fast and the detection is very sensitive. At the same time, the measurement process itself does not consume oxygen, which can effectively improve the accuracy of the experiment compared to the Clark electrode measurement method.

The measuring sensor has been calibrated before leaving the factory and does not require frequent calibration.

Fluorescence quenching technology

Coating of special fluorescent substance on fiber optic oxygen sensor，The presence of oxygen in the sample,Fluorescent substances can be oxidized, or due to the paramagnetism of oxygen molecules, the crossing between systems can be promoted, causing excited singlet fluorescent molecules to transition to triplet states, resulting in fluorescence quenching. The higher the oxygen partial pressure, the stronger the fluorescence quenching.

Model Selection

This introduction and parameters are basic product information and may lag behind product updates. Please contact our company for specific parameters.