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Mobao (Xiamen) Biotechnology Co., Ltd

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    Room 406, Qiangye Building, No. 98 Xiangxing Road, Torch High tech Zone, Xiang'an, Xiamen City, Fujian Province

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TNF-α (80-235aa), Mouse

NegotiableUpdate on 05/06
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Overview

TNF-α (80-235aa), Mouse:Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease.

Product Details

产品简介
SpeciesMouse
Protein Construction
TNF-α (Leu80-Leu235)
Accession # P06804
Purity> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level< 0.2 EU/μg of protein by gel clotting method
Biological ActivityED50 < 0.05 ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells, corresponding to a specific activity of > 2.0 × 107 U/mg.
Expression SystemE. coli
Apparent Molecular Weight~17.3 kDa, on SDS-PAGE under reducing conditions.
FormulationLyophilized after extensive dialysis against PBS.
ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & StabilityUpon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.