Supercritical extractor

Production of high-purity phospholipid concentrate from casein powder using ethanol modified supercritical carbon dioxide

Ethanol modified supercritical carbon dioxide (SC-CO)₂）A food grade green extraction method based on extraction was used to extract phospholipids from casein powderSupercritical extractionTemperature (50 and 60 ℃)Supercritical fluid extractor SFT-120XW-EThe effects of pressure (30 and 40 MPa) and ethanol concentration (10, 15, and 20%, wt/wt) on total lipid yield and phospholipid content. Within the scope of the study, the effect of ethanol concentration on total lipid yield and phospholipid content is more significant than the effects of temperature and pressure. Under the conditions of 60 ° C, 30 MPa, and 15% (wt/wt) ethanol, the highest recovery rate of phospholipids was achieved, with a total lipid yield of 6.3% (wt/wt). Among them, 49% (wt/wt) of phospholipids were composed of dihydrosphingomyelin (5%), sphingomyelin (24%), phosphatidylethanolamine (22%), phosphatidylSIANSUAN (2%), phosphatidylinositol (3%), and phosphatidylDANJIAN (44%). SC-CO modified by Folch method and ethanol₂extractionThe obtained triglycerides have similar components. Through continuous SC-CO₂Pure extraction and ethanol modified SC-CO₂Extract and separate non-polar lipids from the first fraction, thereby concentrating phospholipids from the second fraction. This sequential extraction resulted in a high concentration of phospholipid extract (76%, wt/wt). To our knowledge, this is the highest phospholipid content in casein powder. Therefore, utilizingSupercritical extractorThe obtained phospholipid rich extract can be used as a food grade emulsifier with potential health promoting effects for the development of functional foods.