Succinate dehydrogenase (SDH) test kit; Visible spectrophotometry

Basic Information

Product Name:Succinate dehydrogenase (SDH) test kit; Visible spectrophotometry

Specifications50 tubes/48 samples

Measurement significance

SDH（EC 1.3.5.1）Widely present in animals, plants, microorganisms, and cultured cells.SDHIt is a marker of mitochondriaZhienzyme, a membrane-bound enzyme located on the inner membrane of mitochondria, is one of the hubs connecting respiratory electron transfer and oxidative phosphorylation.In addition, it provides electrons for the respiratory chain of various prokaryotic cells.

Measurement principle

SDH Catalytic dehydrogenation of succinic acid to produce fumaric acid, and the removed hydrogen is passed through phenazine dimethyl sulfate（PMS）Transfer restoration 2,6-Dichlorophenol indophenol（DCPIP）And in 600nm There is a characteristic absorption peak at this point, which is passed through 600nm Changes in absorbance, measurement 2．6-DPIPThe restoration speed represents SDH Enzyme activity.

Equipment and supplies that need to be self provided

Visible spectrophotometer, water bath, desktop centrifuge, adjustable pipette1 mLGlass colorimetric dish, mortar, ice, and distilled water.

Composition and preparation of reagents

Reagent 1:50mL×1 Bottle,-20℃保存；Reagent 2:10mL×1 Bottle,-20℃ storage; Reagent 3:1mL×1 Support,-20℃保存；Reagent 4: Liquid 5mL×1 Bottle,4℃保存；

Reagent 5: Powder×1Support,4Store at ℃, add before use2mLDistilled water, unused reagents still 4℃保存；Reagent 6: Powder×1Support,-20℃ storage; Add before use2mLDistilled water, unused reagents 4℃保存。

Advantages Introduction:
1Over a thousand ready to use carrier strains are available in stock.
2Schools and research institutions support cash on delivery.
3The product has a high demand and can be customized with large packaging.
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Ordering Notice:

1. The quotation includes the general ticket shipping cost..

2. Research institutions support shipping first and payment later

3. More than 10000 types of chemical reagents are in stock, over 3000 types of standard products are in stock, and over 2000 types of ready to use reagents and dye solutions are in stock for convenient one-stop procurement. We also act as agents for imported brands such as Sigma, AMResco, American Herbal Medicine, ABCAM Antibody Kit, CST, Santa, etc.

4. Professional customer service personnel provide answers.

5. Ultra low discounts, saving every penny of research funding!

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China Pharmaceutical University, Ocean University of China, Fujian Tea Research Institute, Peking University, Fudan University, Guangzhou Medical College, Shandong University, Zhejiang University, Zhejiang University of Technology and other institutions.

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