Subcellular resolution spatial multi omics imaging analysis system

In situ analysis of cells in a real organizational environment

In situ analysis

XeniumIn situ analysis technology directly detects and preserves hundreds of tissue slicesRNAThe cellular localization of the target does not require conventional sequencing techniques to assist. This provides researchers with a detailed gene expression pattern map without sacrificing resolution or target quantity.

XeniumIn situ analysis technology utilizes targeted gene specificityCyclicable probes and targetsRNAHybridization, followed by in situ rolling ring amplification using a circular probe, amplifies gene specific spatial tag sequences that can be hybridized with fluorescent labeled probes. inXeniumMultiple rounds of hybridization of fluorescently labeled probes are performed on the analyzer. In each hybridization reaction, the fluorescently labeled probes hybridize with gene specific spatial tag sequences. The images are scanned using a high-resolution microscope to detect the position of each fluorescent probe, which is then removed. Continuous rounds of fluorescence probe hybridization, imaging, and removal will generate a fluorescence color "on" and "off" pattern at each specific probe hybridization location, forming a combination of fluorescence signal characteristic barcodes that reveal the characteristics of each cell and position within the tissueRNAIdentity information. futureXeniumIt can also detect within the same tissue sectionRNABy analyzing protein expression levels, complex and precise expression patterns in tissues can be achieved.

passXenium in situ analysis is used to construct spatial transcriptome profiles.Fresh frozen orFFPEOrganize slice placementinOn Xenium glass slides. Process the slices, obtain RNA, and label it with a recyclable gene specific DNA probe. Subsequently, the probe is connected to generate a circular DNA probe that can be enzymatically amplified. Place the glass slide in a Xenium analyzer for multiple rounds of hybridization, imaging, decoding, and data processing using fluorescent probes.

Application scope and research field

XeniumIn situ analysis technology helps researchers determine the gene and protein expression patterns of cells at specific locations in their original tissues, obtaining detailed spatial information. This technology will facilitate the rapid development of research in multiple important application fields, including:

oncology

Reveal the spatial characteristics of heterogeneity in the tumor and immune microenvironment, and identify biomarkers with potential therapeutic effects.

Immunology

Characterizing how the immune system and spatial localization respond to infectious diseases, allergic inflammation, and autoimmunityDisease.

neuroscience

Reveal the spatial expression patterns of complex neural tissues in response to diseases and injuries.

Product Specifications