Smooth muscle cells of rat pulmonary artery

NegotiableUpdate on 05/06

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Overview

Immunofluorescence identification of smooth muscle cells in rat pulmonary artery revealed that the pulmonary artery originates from the right ventricle, runs diagonally to the left, upper, and rear before the aorta, and divides into left and right pulmonary arteries below the aortic arch, entering the lungs through the pulmonary hilum. Since the pulmonary artery is connected to the right ventricle that transports venous blood, although it is an artery, it transports venous blood. Smooth muscle cells of blood vessels connect with each other to form a tubular structure; Functionally, it can generate continuous contraction, allowing organs to resist the applied load and maintain their original shape.

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　　Smooth muscle cells of rat pulmonary arteryDetailed description:

The pulmonary artery originates from the right ventricle and runs obliquely to the left, upper, and rear before the aorta. It divides into the left and right pulmonary arteries below the aortic arch and enters the lungs through the pulmonary hilum. Since the pulmonary artery is connected to the right ventricle that transports venous blood, although it is an artery, it transports venous blood. Smooth muscle cells of blood vessels connect with each other to form a tubular structure; Functionally, it can generate continuous contraction, allowing organs to resist the applied load and maintain their original shape. The calcium channel surface expressed by this cell contains ICAM-1 and VCAM-1, which are involved in vascular wall inflammation response. Smooth muscle cells of pulmonary arteries cultured in vitro exhibit spindle shaped, star shaped, or irregular shapes.

These cells not only participate in maintaining the normal tension and structure of blood vessels, but also play a role in the pathological and physiological processes of various lung diseases, especially in the development of pulmonary arterial hypertension (PAH).

In terms of morphology, pulmonary artery smooth muscle cells are spindle shaped, irregular shaped, triangular or fan-shaped, with oval shaped and centered nuclei. Under in vitro culture conditions, these cells can adhere to the wall and form single-layer or multi-layer structures, exhibiting a fibroblast like morphology. Their growth characteristics can be maintained through specific cell culture media and conditions, such as using media containing FBS, growth additives, penicillin, and streptomycin, and culturing in air containing 5% CO2.

The function of pulmonary artery smooth muscle cells includes participating in the inflammatory response of the vascular wall, achieved through the expression of cell surface mediators such as ICAM-1 and VCAM-1. In addition, they are also target cells for most important arterial diseases. Pathologically, the abnormal apoptosis and proliferation imbalance of these cells are key to pulmonary vascular remodeling and closely related to the occurrence and development of pulmonary arterial hypertension. Research has shown that hypoxic conditions can promote the proliferation of pulmonary artery smooth muscle cells, which may involve multiple signaling pathways, such as the TLR9 JNK signaling pathway.

In research, pulmonary artery smooth muscle cells are often used to study the pathogenesis of pulmonary vascular diseases and develop new treatment methods. For example, by studying the effects of hypoxia on these cells, scientists can better understand the pathological process of pulmonary arterial hypertension and explore potential treatment strategies. In addition, these cells can also be used for drug screening and toxicity testing to evaluate the impact of new drugs on the pulmonary vascular system.

　　Smooth muscle cells of rat pulmonary arteryCharacteristics:

1) The organization is derived from normal pulmonary artery tissue of experimental animals.

2) Cell identification: Smooth muscle actin (α - SMA) immunofluorescence staining was positive.

3) After identification, the purity of the cells was found to be higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: spindle shaped cells, adherent culture.

Transportation mode:

Low temperature: (1) Transport in 1mL cryovials packaged with dry ice. After receiving, store in a -80 degree freezer overnight and transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the cryovial cap has fallen off, is damaged, or the cells are contaminated, please contact us immediately.

At room temperature: (2) T25 bottles of revived surviving cells will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.

Bio-Safety:

1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal.

2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells.

Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.