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Shanghai Anwei Biotechnology Co., Ltd
Mouse primary aortic endothelial cells
NegotiableUpdate on 05/06
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Overview
Mouse primary aortic endothelial cells, also known as aortic endothelial cells, form the inner wall of the aorta and are continuously affected by blood flow shear stress. Under the action of shear stress, endothelial cells secrete different endothelial factors, which in turn affect vascular contraction and growth. Aortic endothelial cells also regulate the expression of cell adhesion molecules to control and precisely regulate inflammatory responses and tissue fibrosis. Primary aortic endothelial cells cultured in vitro can effectively help researchers to study the mechanism of endothelial dysfunction and the pathogenesis of atherosclerosis and other diseases.
Product Details
Mouse primary aortic endothelial cellsDetailed description:
Bone marrow mesenchymal stem cells are bone marrow stromal stem cells that not only provide mechanical support to hematopoietic stem cells (HSCs) in the bone marrow, but also secrete various growth factors (such as IL-6, IL-11, LIF, M-CSF, SCF, etc.) to support hematopoiesis. Bone mesenchymal stem cells (BMSCs) have the potential for multi-directional differentiation and can promote the regeneration of mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, fat, and matrix.
In the bone marrow, BMSCs account for 0.001% to 0.1% of the total number of nucleated cells, and their content is extremely low. However, due to the large number of seed cells required for tissue engineering, the technical difficulty of isolating BMSCs from rodent bone marrow has limited the progress of many experiments. The isolation and cultivation of BMSCs with high purity, strong vitality, and uniform biological characteristics in vitro is crucial for tissue engineering and in vivo and in vitro experiments of cells.
Mouse primary aortic endothelial cells are a type of cell isolated from the aortic tissue of adult mice. They form the inner wall of blood vessels and play a critical role in maintaining vascular function, regulating blood flow, and participating in the pathogenesis of cardiovascular diseases. These cells are capable of secreting various endothelial factors, controlling vascular tone, and participating in regulating the expression of cell adhesion molecules to control inflammation and tissue fibrosis. In terms of morphology, they usually exhibit epithelioid and polygonal shapes, grow adherent to the wall, and have high proliferation and migration abilities. Under the condition of in vitro culture, it can maintain its biological characteristics, which provides an important cell model for studying the mechanism of endothelial dysfunction, the pathogenesis of cardiovascular diseases such as atherosclerosis, and developing new therapeutic methods. The purity of cells is usually identified by immunofluorescence staining and other methods, such as positive expression of CD31 or vWF, with a purity of over 90%. The applications of these cells in research include mechanisms of cardiovascular disease, drug screening, and pathological studies in multiple fields.
Characteristics:
1) The organization is derived from normal bone marrow blood tissue of experimental animals.
2) Cell identification: CD44 immunofluorescence staining is positive.
3) After identification, the purity of the cells was found to be higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
5) Cell growth mode: spindle shaped cells, irregular cells, adherent culture.
Transportation mode:
Low temperature: (1) Transport in 1mL cryovials packaged with dry ice. After receiving, store in a -80 degree freezer overnight and transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the cryovial cap has fallen off, is damaged, or the cells are contaminated, please contact us immediately.
At room temperature: (2) T25 bottles of revived surviving cells will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.
Bio-Safety:
1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal.
2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells. Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.
