Slow virus packaging experimental service

Slow virus packagingExperimental services

Slow virus packaging

It is a type of viral vector modified from the human immunodeficiency virus (HIV), with an RNA genome and the ability to infect both dividing and non dividing cells. It is a powerful tool for introducing exogenous genes and is widely used in research on gene overexpression and RNA interference in cell lines.

Experimental Procedure

1. Gene synthesis and construction of viral vectors;

2. Cell culture and lentiviral packaging after transfection:

3. Harvest virus liquid and purify the virus; .

4. Virus titer detection;

5. Evaluate the infection effect using WB and qPCR.

Experimental cycle and delivery standards

1. Experimental period: 2-6 weeks

2. Delivery standards: Base information, sequencing validation results, lentivirus, infection images, qPCR and WB validation results, experimental report (experimental steps, reagents, instruments, etc.)

Information to be confirmed

1. Genetic species and ID number

2. Gene sequence information

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