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Shanghai Yanjin Biotechnology Co., Ltd
RNA antisense purification (RAP) experimental service
NegotiableUpdate on 05/06
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Overview
Service Name: RNA antisense purification (RAP) experimental service $r $n Provider: Shanghai Yanjin Biotechnology $r $n Specification: RAP $r $n Price: 2000 yuan $r $n Cycle: 1-3 months
Product Details
RNA antisense purification (RAP) experimental service
Experimental significance
Exploring the interaction between post transcriptional RNA and protein, as well as the dynamic changes of post transcriptional regulatory networks, can understand the dynamic changes of post transcriptional regulatory networks such as malignant tumors and genetic diseases, and can be applied to pathological diagnosis and scientific research.
Experimental Principle
RNA Antisense Purification (RAP) Design - A complementary trans nucleotide probe is designed to target RNA and biotinylated. The probe is used to pull down the target RNA and capture the proteins that interact with it. Then, RAP is purified using streptavidin labeled magnetic beads and labeled with Real
Tme RT-PCR. WB、 Further identification using second-generation sequencing or mass spectrometry.
Experimental Procedure
1. Probe design
2. Preparation of test samples (cells, tissues, or test RNA and proteins, etc.)
3.Hybridization of biotin labeled probes with RNA
4.RAP
5.Separate RNA and protein for identification and analysis.
Results Display
Experimental cycle and delivery standards
1. Experimental period: 1-3 months
2. Delivery standards: RAP identification result report, analysis report, experimental report (reagents, consumables, experimental steps). Raw data, etc
Information to be confirmed
1. Target genes, species, and ID numbers.
2.Target gene sequence
3.Sample size and analysis requirements
Partial experimental outsourcing services
