Semen analysis system

NatureGene Sperm Tracker CASASemen analysis systemMulti species version (research work on humans, pigs, horses, cows, sheep, chickens, etc.) HST computer-aided semen quality evaluation system.

The new generation product jointly developed by multiple species HST companies is evaluated by numerous experts, strictly following the regulations of WHO2007 version on CASA system, and utilizing modern computer recognition technology and image processing technology to comprehensively quantify the dynamic and static characteristics of sperm. Its product has been evaluated by experts from the United States, India, Russia, and other countries and is at a high level. This system includes sperm motility analysis, morphological analysis, and fully considers the practical needs of grassroots clinical practice in its design. It has stable performance, friendly human-machine interface, intuitive and clear operation, and users can master the operation methods without special training.

Semen analysis systemUsed for clinical semen testing, it can greatly improve the level of clinical testing and is widely used in urology, urology, family planning, eugenics research, and other professional fields. It also has extensive application value in certain fields, such as the Institute of Endangered and Rare Animals in the Institute of Biology, animal husbandry research departments, and artificial insemination stations.

At present, there should be English, Chinese, French, Japanese, and Russian versions available.

Main functions:
Computer automation detection: curve motion - average curve motion speed, total number of curve motion sperm, total number of curve motion active sperm, curve motion activity rate
Linear Motion - Average Linear Motion Speed, Total Linear Motion Sperm Count, Total Linear Motion Sperm Count, Linear Motion Activity Rate
Average path motion - average path velocity, average path sperm count, average motile sperm count, average motile sperm motility
Sperm motility polarization statistics - * fast forward motility rate, b-grade slow forward motility rate, c-grade non forward motility rate, d-grade extremely slow or immobile rate
精子密度
Sperm lateral swing amplitude
Sperm motility
Sperm whipping frequency
linear velocity
Average movement angle of sperm
Linear motion sperm count
Linear motility rate, total sperm count
Testing time:
Sperm density, sperm motility, sperm movement trajectory, and sperm movement distribution map;
Average curve, speed curve, total number of sperm, motility curve, total number of sperm, motility curve, motility rate
Average straight-line velocity, total number of sperm in straight-line motion, total number of sperm in straight-line motion, and motility of sperm in straight-line motion
Average path speed, average path sperm count, average motile sperm count, average motile sperm motility
Sperm motility grading: * fast forward motility, b slow forward motility, c non forward motility, d extremely slow or immobile motility
Sperm lateral swing amplitude, sperm swinging, sperm whipping frequency, linear motion rate, total number of sperm
Linear velocity, average movement angle of sperm, number of sperm moving in a straight line ◎ Other components of semen - epithelial cells, red blood cells, white blood cells
Abnormal sperm count - head abnormal sperm count, tail abnormal sperm count, body abnormal sperm count, mixed abnormal sperm count
Sperm abnormality rate:
Sperm appearance, sperm viscosity
Semen volume, detection dilution ratio
Sperm odor and sperm extraction method
Sperm pH value, date and time of sperm retrieval
Abstinence time, patient's basic data
Image processing: The software supports basic image processing functions such as calibration, length and area measurement, color filtering and pseudo color, brightness contrast adjustment, text and area annotation, etc
Report: Using the WYSIWYG report format, the operator is like writing on an actual report form, and the input is printed at the same location as the input
Custom report format: The software report format can be freely modified, allowing for the adjustment of any image and the placement of any text in the report

Main technical indicators:
The allowed number of samples to be collected is 1-1000 (or more)
Range of detection speed (um/s) 0-180
The number of captured images (frames) 0-75
Particle diameter resolution (um) 3-50
Collection and analysis time (seconds) 1-5 or longer
Number of image acquisition groups (groups) 1-10

Natural Gene Co., Ltd.:

The NATUREGENE HST sperm quality analysis system is a well-known computer-aided analysis (CASA) system. The use of * computer technology for automated quantitative detection and analysis of sperm density, vitality, viability, and movement trajectory characteristics has been published in various well-known academic journals. The entire HST system consists of microscopic magnification equipment, digital rapid capture equipment, software packages, and workstations. This system is used for clinical semen examination and can greatly improve the level of clinical testing. It is widely used in urology, urology, obstetrics and gynecology (in vitro fertilization), family planning, and eugenics research in hospitals. This system also has wide application value in certain professional fields, such as biological research, animal research (pigs, cows, sheep, pandas, fish, etc.), etc. The fully automated analysis software package MMC is used for semen quality analysis based on the recommended parameters of the World Health Organization (WHO). This software package is based on our professional database system and has specialized sperm analysis procedures.

advantage

1. Can quickly, accurately, and objectively detect various parameter indicators of sperm motility (static) state.

2. The range of sperm density detection is wide, and the sample does not need to be diluted.

3. It can filter out various impurities that are different from sperm morphology to ensure the accuracy of detection.

4. Non destructive testing sample pool ensures both the natural movement of sperm and single-layer sampling.

5. The system software features not only comprehensive detection of sperm motility, but also morphological detection of abnormal sperm staining, various pathogenic microorganisms, blood cells, etc.

6. The computer with * function can quickly store various medical records and images, and can be called up for viewing, playback, and printing at any time.

7. The WINDOWS operating interface allows for direct human-machine dialogue with multiple settings and report formats, meeting different user needs and application scopes.

8. The system has good intuitiveness, providing doctors and patients with more intuitive and vivid observation methods. 

Main features of the software:

Professional digital fast imaging equipment for capturing images and AVI format videos

Store text data, images, and videos in the built-in professional database. Enable sperm analysis applications to enjoy all the features of the database. This database is a tool for archiving patient data, analyzing results, videos, and images; A report with fast and flexible search, including images, charts (or any other required elements). Therefore, our database can meet the needs of almost all laboratories or research institutions

Automatically analyze the sperm concentration and motility of natural samples according to WHO recommended standards

Perform automatic morphological analysis on stained samples according to strict Krueger standards

For special research or other individual tasks, manual measurements are used

Why buy HST?

Working with a camera is more comfortable than observing with an eyepiece (prolonged observation with an eyepiece can cause eye fatigue)

Using HST to analyze motility can save the video on a hard drive so that it can be analyzed again at any time, otherwise it must be done while the sperm is still alive.

Morphological analysis is more accurate. The software will evaluate all the cells captured.

Using automated software for sperm analysis can reduce human factors. For example, visual assessment of sperm heads is always complex, but with HST, you can obtain objective quantitative data such as head length, width, area, and shape factor. In the analysis of motility, if you know its speed and precise trajectory, the evaluation of sperm motility becomes more objective. But the conclusion after * is always made by the customer, and you can correct the automatic analysis results.

Using computer-aided sperm analysis makes it easier to train new laboratory employees and makes their work quality more reliable

The MMC professional database provides unlimited recording capacity and can capture an unlimited number of views to obtain all necessary patient information, and can quickly retrieve them

You can choose a suitable work location on the local network, such as placing the database on a server (which is protected by a firewall and has an automatic backup program). Even when used on a personal computer, HST's two-stage deletion program can protect your data. If you delete a record, it will first go to the system's recycle bin and can also be restored.

Choosing the appropriate database region type helps you prevent typing errors in the database (text cannot be entered within a number or time region). A dropdown list will make input faster and more accurate, especially for repetitive information.

Patients will prefer reports with greater information content, including explanations, standard reference values, images (as well as other user added information).

Why sell HST?

HST is a computer-aided sperm analysis system (CASA). By becoming our distributor, you can enjoy an integrated system: providing matching microscopes, sperm counting plates, consumables, dyes, and many other supporting equipment, which can provide comprehensive support for users. We are more than happy to share information with you. We provide consulting services on how to build a complete CASA system and offer comprehensive customer support, including installation and training.

Because we are a professional image analysis software development team, we will not stop moving forward: we continuously improve and upgrade our software. The development of software will not end, you can request the addition of features according to the needs of users.

Sports routine:
Semen concentration assessment (M/ml) and motility analysis were performed on AVI video (using your imaging device to capture real-time images into computer memory or hard drive). The analysis is strictly conducted in accordance with the requirements of the WHO laboratory manual, detecting the contact between human semen and sperm cervical mucus (test). （《WHO laboratory manualfor the examination of human semen and sperm-cervical mucus interaction》）

The following parameters will be calculated:

VCL=curve velocity (micrometers/second), the average curve velocity of the sperm head moving along the actual curve trajectory, observed in two dimensions under a microscope

VSL=Linear velocity (micrometers/second), the average curve velocity of the sperm head moving along a straight trajectory (the line connecting the starting and ending points)

VAP=average path velocity (micrometers/second), the average curve velocity of the sperm head moving along the actual average path, which is calculated by smoothing the actual path

BCF=beat cross frequency (beats/second), the average frequency at which the sperm curve trajectory crosses the average path

LIN=Linear, the linearity of a curved path

STR=straightness, linearity of average path

Elongation=elongation rate, based on the above parameters, the motility of each sperm can be divided into four levels: A, B, C, D (WHO):

A=Rapid Movement Type

B=Slow or sluggish mobility

C=No mobility

D=Fixed

In addition, the software can evaluate concentration:

white blood cell

Immature germ cells

Circular cell

By pointing the mouse cursor to a trajectory, you can see the precise parameter values of the selected sperm, and in a pop-up window, you can see the classification results. The video can be replayed, backtracked, and played every frame. It can be saved without compression (in full quality) or compressed using software on the computer. You can also adjust the detection threshold or change the classification criteria.

The number of sperm detected and tracked will be displayed on the screen, and it can be checked whether enough cells have been tracked (WHO reference is 200). When the analysis is completed, the results of automatic classification and manual counting are automatically transmitted to the database.

Morphological routines:

Sperm head morphology is an important criterion for accurate diagnosis. The software is set up for analyzing static images, and the samples need to be stained with Diff Quik dye according to strict Krueger standards. We chose Diff Quik, which is a rapid staining method for sperm. Through Diff Quik, the sperm head is stained light blue in the acrosome region and dark blue in the posterior acrosome region, which is a good foundation for precise graphic analysis. The following parameters were measured for each sperm:

Area， Head area

FFC=circular shape coefficient, similarity between sperm head and circle

Perimeter， The circumference of the head

Brightness， brightness

ELL-B=elliptical long axis of sperm head contour, i.e. the length of the sperm head

ELL_S=elliptical short axis of sperm head contour, which is the width of the sperm head

Elng=elongation of sperm head

FFE=Oval Shape Coefficient, the similarity between sperm head and ellipse

Acrosome=percentage of acrosome region

The software automatically classifies sperm into normal and head pathology based on head parameters. You can easily manually correct the results and develop other abnormalities (tail pathology, neck pathology) if you cannot obtain good staining images for automatic detection. For example, due to incorrect sample preparation, there is a special tool that can manually animate cell contours.

Nowadays, it is common to record the number of morphological sperm defects divided by the number of defective sperm, which is called the teratozoospermia index (TZI). After the analysis is completed, TZI is automatically calculated. The TZI value should be between 1.00 (each malformed sperm has only one defect) and 3.00 (each malformed sperm has head, neck, and tail defects).

report

The analysis results can be printed out in HTML format. The report is generated based on the database and saved in the corresponding database records. There are the following features: grouping of data, ability to change the appearance of data order, display graphics, etc. In fact, any requirement can be achieved through any HTML editor (such as MS Word). If there are special needs, we can help you adjust the report.

Hardware requirements:

Because HST is a part of computer-aided design, you need to consider some special hardware requirements. Generally speaking, collecting all system components by oneself is not complicated, but we highly recommend obtaining all components through local agents.

Computer hardware requirements:

Windows XP, Vista, Windows 7

At least 1 Gb of RAM memory
Microsoft. NET Framework v2.0 or higher This is the operating environment of the program. If you don't see it in the installation program list (Start Control Panel Add/Remove Programs), please download the Microsoft web page from the Microsoft website

counting board

Sports analysis requires special counting plates with fixed depths such as Leja, Makler, MicroCell, Cell Vu, etc. These counting boards provide standard volumes and sperm are distributed within a single layer of aggregation plane.

Practice has shown that Leja counting slides are the preferred option for CASA.

Leja provides a wide range of disposable counting plates for sperm analysis. Slips with 2 or 4 chambers, with chamber depths of 10 or 20 micrometers. Leja slides have a standard low abundance quantitative series that can detect low concentrations of sperm. Its diagnostic significance is that you can choose from IUI, IVF, or ICSI to determine the threshold. Leja slides are very easy to use, and new members of the laboratory can quickly master how to use them.

Makler sperm counting plate (chamber) is a convenient device used for rapid and accurate sperm counting, motility, and morphological evaluation, using undiluted samples.

It consists of two parts (Figure 1):

1. The main part below consists of a metal substrate (A) and two handles (H). There is an optical flat glass flat disk (D) at the center of the substrate, on which the sample is placed. There are four needles (P) around the flat plate. Their * is 10 microns higher than the flat plate.

2. The upper part is covered with glass (C) surrounded by a metal ring. There is a 1 mm2 grid in the center of its lower surface, divided into 100 grids, each measuring 0.1 X 0.1mm. When the cover glass is placed on four needles, the space enclosed by 10 squares on a row is one millionth of a milliliter. Therefore, the number of sperm heads in the 10 squares represents their concentration in millions/mL.

Microscope:

Three eye upright transmission light laboratory grade microscope, which can be equipped with a camera.

The camera interface conforms to the size of the image detector. We recommend using 1x interface for 1/2 "detector

You can choose between bright field or phase difference. The software can recognize dark targets on a bright background or vice versa. So dark fields can also be used.

The 20X objective lens is used for mobility. If a reusable counting board, such as Makler, is used, a long working distance lens (approximately 1.8 millimeters) is required. If a disposable counting board, such as Leja, is used, it is not necessary.

100X oil microscope for morphology

10X lens for observation

camera

We have found a solution for the sperm image analysis task. We highly recommend ordering our USB digital high-speed camera together with MMC software. Because:

1/2 "high-quality detector. Our camera has excellent balance in detector performance, speed, and cost-effectiveness.

USB connection allows you to connect the camera to any desktop or laptop computer. *The requirement is a good modern chipset to maintain a complete frame rate per second.

For sperm motility, images and videos with a resolution of 800X600 and 60fps are directly analyzed in computer memory without being saved on the hard drive. This reduces the requirements for computer configuration. If using a third-party camera, you must first save the video on the hard drive, which slows down the analysis speed.

For sperm morphology, analyzing static color images with a resolution of 1280X1024 directly in computer memory also improves performance.

The camera can record the settings applied in motion and morphology, and save them in its own memory. So if you replace the computer, there is no need to reset it

For dealers: Cameras are provided in small packages. Save a lot of space and shipping costs

However, you can decide to use the existing equipment on your own. Please note the following requirements:

DirectShow driver to enable software to recognize cameras

For sportiness: It can record 50fps videos without loss at a resolution of 640X480

For morphology: good color reproduction at low resolution in VGAzui

Sample preparation

locomotion

Sperm concentration is measured using a special counting plate, which has a fixed depth to provide a standard volume, and the sperm are distributed in the same focal plane and can move freely. The tracked sperm is always in the focal plane. The sperm counting plate can use undiluted samples. Be careful when mixing sperm samples during preparation to avoid foaming. If the sample is highly viscous, it can be aspirated several times with a syringe to make it uniform.

morphology

In order to obtain high-quality sperm staining images for morphological evaluation, semen slurry should be diluted first and removed after centrifugation. The sperm balls are resuspended within the volume to achieve the highest sperm concentration, but not exceeding 80 x 10 ^ 6/ml. 0.2 to 0.5ml of semen, depending on semen concentration, is diluted to 10ml with physiological saline at room temperature. The test tube is centrifuged at 800g for 10 minutes and the majority of the suspension settles. The ball is suspended in the remaining saline solution, usually 20-40ul, and gently tilted through a test tube. Then hang 5-10 ul of this
Floating liquid prevention is performed on a glass microscope slide, and the droplets need to be spread out on the slide using a pipette for staining, as described below.

Staining of sperm morphology.

(a) Feather method dilutes semen. The droplets spread along the back edge of the tilted glass slide and are pushed forward to the plane of the slide to form a layer.

(b) Wash the sample using the pipette method. A droplet of suspended matter diffuses onto the surface of a glass slide through a horizontal pipette.

Prepare the coating. Make the least two coatings from fresh semen samples to repeat the experiment. Firstly, the bottom of the slide should be cleaned, washed with 70% ethanol and dried before placing small drops (5 to 20ul) of semen. If the semen concentration is greater than 20X10 ^ 6/ml, 5ul can be used; if the semen concentration is lower than 20X10 ^ 6/ml, 10 to 20ul should be used. The feather method uses the edge of another slide to spread the droplets along the surface, but care must be taken not to make the coating too thick. Feather technique works well when viscosity is low, but it is usually not suitable for viscous semen. As an alternative, a drop of semen can be prevented from being in the middle of the slide, and then another slide can be placed upside down to spread the semen between them: then gently pull apart both slides to make two coatings at the same time.

The software was adjusted using the Diff Quik staining method. Please follow your Diff Quick instruction manual.

This applies to research work on various species.