Seahorse Cell Energy Metabolism Detection Service

Project advantages:

simultaneous detectionOCR and ECAR.

Micro chamber detection with precise results.

Low cell demandGreatly reduce the consumption of target test drugs and cells, and lower research costs.

real-time detectionDo not damage the sample.

Up to four drugs can be added,real-timeMonitor drug efficacy.

Suitable for primary cells, adherent cells, and suspended cells.

Intelligent analysis, automatic acquisition of experimental data and curves after sequencing is completed.

Application fields:

cancer researchThe energy metabolism of cancer cells differs from that of normal cells, including enhanced glycolysis and weakened oxidative phosphorylation.Seahorse cell energy metabolism detectionIt can be used to evaluate the glycolysis and oxidative phosphorylation activity of cancer cells, study the energy metabolism characteristics of tumor cells, explore the mechanism of cancer development, and provide guidance for the development of tumor therapies.

Research on metabolic diseases:Metabolic diseases such as obesity, diabetes and fatty liver are closely related to abnormal cellular energy metabolism.Seahorse cell energy metabolism detectionIt can be used to evaluate the energy metabolism of adipocytes, hepatocytes, and pancreatic islet cells, study the pathogenesis of these diseases, and search for new therapeutic targets.

Neuroscience research:Neural cells require a large amount of energy to maintain their function and activity.Seahorse cell energy metabolism detectionIt can be used to evaluate the energy supply and metabolic status of nerve cells, study energy metabolism defects in neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, and investigate the effects of drugs on energy metabolism of nerve cells.

Immunological research:Immune cells such as lymphocytes and macrophages have special requirements for energy metabolism.Seahorse cell energy metabolism detection can evaluate the energy metabolism activity of immune cells, study the metabolic pathways and regulatory mechanisms of immune cells, and explore immune regulation and treatment strategies for immune related diseases. In addition,Seahorse cell energy metabolism detectionIt can also be applied in various fields such as aging, muscle physiology research, cardiovascular disease research, stem cell research, translational medicine, drug screening, etc. It provides a powerful tool for studying cellular energy metabolism and its role in different diseases and physiological processes.

1、 Technical Introduction

The Seahorse Energy Metabolism Analysis System is a technology platform based on live cell metabolism detection, mainly used to monitor the energy metabolism status of cells. It has the characteristics of label free, real-time dynamic, and high sensitivity, and is an important detection method for cell energy metabolism research. The core detection parameters include oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), which reflect mitochondrial respiratory function and glycolytic activity, respectively. OCR refers to the rate at which cells consume oxygen. Cells are placed in specific oxygen consuming reagents and their OCR is calculated by measuring changes in dissolved oxygen content in the surrounding culture medium, further evaluating mitochondrial respiratory function. ECAR refers to the extracellular acidification rate, in which cells are placed in a specific acidification reagent and the changes in pH in the culture medium are measured to calculate ECAR and evaluate the metabolic adaptability of cells under stress.

2、 Service features

1. One stop service: providing one-stop services from cell culture, drug treatment to Seahorse energy metabolism detection.

2. Detailed report: Provide a complete experimental report and detailed data analysis, promising the authenticity and reliability of the data.

3、 Sample delivery

Sample shipping:cell count≥ 10 ^ 6, T25 bottles of live cells are sent out at room temperature (suitable for close range transportation) or frozen cells are sent out with dry ice (suitable for long-distance transportation), and drugs or other reagents are sent out according to the storage conditions.