Saffron solid green dyeing service

The safranin green staining service is suitable for staining the roots, stems, and leaves of higher plants. The staining results show that lignified cell walls and nuclei appear red, while thin-walled cells and cytoplasm appear green. In morphological studies involving articular cartilage and subchondral bone, it is often necessary to use a combination of multiple dyes to display the histological structure of the cartilage using safranin green staining (cartilage). Among them, safranin O-fast green staining, which originated in the 1960s, is highly favored for its ability to visually reflect the structure of articular cartilage, subchondral bone, and bone tissue. Cartilage appears red, while osteogenesis appears green.

(1) Plant materials should be selected as little as possible without damaging the plant body or the required parts;

(2) When using animal materials, anesthesia should be applied to the animals, or the necessary tissues should be quickly removed after killing the animals;

(3) The material must be fresh, which is particularly important for conducting cell biology research. Living tissue blocks should be harvested as much as possible and immediately fixed with fixative, with a volume ratio of 10:1 between fixative and tissue blocks;

(4) When cutting materials, the knife should be sharp to avoid damaging the cells due to squeezing;

(5) The cut material should be small and thin, making it easy for the fixing liquid to quickly penetrate into the interior. Generally, the thickness should not exceed 3mm and the size should not exceed 5 × 5m ㎡;

Attention to safranin green staining service: Tissues that require paraffin embedding should generally not be frozen. If the tissue has been frozen, it should be directly placed in a fixative when taken out of the refrigerator, and the volume ratio of fixative to tissue block should be 20:1.

Product Introduction: The safranin green staining service, abbreviated as safranin staining, is a staining method used to observe different tissue morphologies in plants. After staining, the lignified and cork like cell walls of the tissue are stained red; Cellulose cell walls stained green; The catheter is dyed red, and the sieve tube is dyed green.

Experimental process of safranin green staining service:

(1) Sample reception: paraffin sections, cell slides, frozen sections

(2) Experimental steps: dewaxing to water - safranin staining - gradient alcohol - solid green staining

(3) Experimental results: Stained glass slides

Precautions for safranin green staining service:

1. Pay attention to the usage cycle of the dye solution.

2. Note that the two colors should not be mixed.