SHP-77 human small cell lung cancer cells

genus:Human Source（Homo sapiens）

source of organization:Lung（Lung）

disease:Cancer（Carcinoma）

age: 54Year old（54 years）

gender:Male（Male）

cell type:Epidermal cells（Epithelial）

Growth characteristics:Wall sticking and suspended growth（Adherent and suspension）

Content:>1x106Cell count

SpecificationsT25Bottle or1mLFreezing tube packaging

Purpose: For scientific research purposes only.

Transportation and storage

Dry ice transportation and recovery of viable cells

（1）1mLFrozen storage tube packaging for dry ice transportation, upon receipt-80After overnight storage in the refrigerator, transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the bottle cap of the freezing tube has fallen off, is damaged, or the cells are contaminated, please contact us immediately.

（2）T25Surviving cells revived from the bottle will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.

Processing after cell reception

1）After receiving the cells,75%Alcohol disinfection bottle wall willT25Bottle placed37Place the incubator at approximately ℃2-3hIf you find any damage to the culture bottle, overflow of liquid, or contamination of cells, please take photos and contact us promptly.

2）Please in4or5XConfirm the cell status under the microscope and take photos of the newly received cells（10×，20(X) Each2-3Please keep a photo of Zhang and the appearance of the culture bottle as a reference for the cell status upon receipt during after-sales service.

3）Note: The transport medium (infusion medium) cannot be used to culture cells anymore. Please use a specialized medium newly prepared according to the instructions for cell culture conditions to culture cells. Suggestions for the first passage after receiving cellsT25culture flask1：2Passage.

1、 Preparation of culture medium and culture cryopreservation conditions:

1）1640culture medium+10%FBScharacterized FBS+1%L-glutamine+1%sodium pyruvate+1%P/Sbispecific antibody

2） Cultivation conditions: Gas phase: Air,95%Carbon dioxide,5%Temperature:37Celsius degree, incubator humidity is70%-80%.

3） Freezing solution:90%serum10%DMSOCurrently in use and equipped.

II Cell processing:

1） Recovery of frozen cells:

Will contain1mLThe freezing tube for cell suspension37Quickly shake and thaw in a ℃ water bath, then add to the solution containing4-6mL dedicatedMix the culture medium evenly in the centrifuge tube. in1000RPMCentrifuge under conditions3-5minDiscard the supernatant and resuspend the cells in specialized culture medium. Then add the cell suspension to the solution containing6-8ml dedicatedIn the culture bottle (or dish) of the culture medium37Incubate overnight at ℃. The next day, observe cell growth and cell density under a microscope.

2） Cell passage: If the cell density reaches80%-90%You can proceed with subculture.