RC+LUC+GFP human B lymphoma cells+LUC+GFP

RC+LUC+GFP human B lymphoma cells+LUC+GFP

Cell characteristics

genus:Human Source（Homo sapiens）

source of organization:Pleural effusion（Pleural effusion）

disease: BCell lymphoma（B-cell lymphoma）

age: 71Year old（71 Years）

gender:Female（Female）

cell type:Lymphoblastic cell like（Lymphoblast-like）

Growth characteristics:Suspended growth（Suspension）

Content:>1x10^6Cell count

SpecificationsT25Bottle or1mLFreezing tube packaging

Purpose: For scientific research purposes only.

Transportation and storage

Dry ice transportation and recovery of viable cells

（1）1mLFrozen storage tube packaging for dry ice transportation, upon receipt-80After overnight storage in the refrigerator, transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the bottle cap of the freezing tube has fallen off, is damaged, or the cells are contaminated, please contact us immediately.

（2）T25Surviving cells revived from the bottle will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.

Processing after cell reception

1）After receiving the cells,75%Alcohol disinfection bottle wall willT25Bottle placed37Place the incubator at approximately ℃2-3hIf you find any damage to the culture bottle, overflow of liquid, or contamination of cells, please take photos and contact us promptly.

2）Please in4or5XConfirm the cell status under the microscope and take photos of the newly received cells（10×，20(X) Each2-3Please keep a photo of Zhang and the appearance of the culture bottle as a reference for the cell status upon receipt during after-sales service.

3）Adherent cells: Cells in37Placed in a ℃ incubator2-3hObserving the growth and adhesion of cells under a microscope, some adherent cells may detach and form clusters due to vibration during express delivery. If the growth density of cells is observed under a microscope, then60%The following can remove the culture medium from the culture bottle (if there are non adherent cells, they need to be recovered by centrifugation and resuspended in the original culture bottle),Add newly prepared specialized culture medium6-8mLContinue to culture in the cell culture incubator. If the cell growth density reaches70%-80%Above, cells can be passaged. During the passage process, if cells shed due to transportation vibrations, they need to be recovered by centrifugation.

4）Note: The transport medium (infusion medium) cannot be used to culture cells anymore. Please use a specialized medium newly prepared according to the instructions for cell culture conditions to culture cells. Suggestions for the first passage after receiving cellsT25culture flask1：2Passage.

1、 Preparation of culture medium and culture cryopreservation conditions:

1）RPMI-1640culture medium+10%FBScharacterized FBS+1%P/Sbispecific antibody

2） Cultivation conditions: Gas phase: Air,95%Carbon dioxide,5%Temperature:37Celsius degree, incubator humidity is70%-80%.

3） Freezing solution:90%serum10%DMSOCurrently in use and equipped.

II Cell processing:

1） Recovery of frozen cells:

Will contain1mLThe freezing tube for cell suspension37Quickly shake and thaw in a ℃ water bath, then add to the solution containing4-6mL dedicatedMix the culture medium evenly in the centrifuge tube. in1000RPMCentrifuge under conditions3-5minDiscard the supernatant and resuspend the cells in specialized culture medium. Then add the cell suspension to the solution containing6-8ml dedicatedIn the culture bottle (or dish) of the culture medium37Incubate overnight at ℃. The next day, observe cell growth and cell density under a microscope.

2） Cell passage: If the cell density reaches80%-90%You can proceed with subculture.

For the passage of adherent cells, the following methods can be referred to:

1. Discard the culture supernatant and use one that does not contain calcium or magnesium ionsPBSMoisturizing cells1-2Next time.

2. join0.25％（w / v）Trypsin-0.53 mM EDTAIn the culture bottle（T25bottle1-2mL，T75bottle2-3mL）, placed in37Digestion in a ℃ incubator1-2Minutes (for difficult to digest cells, the digestion time can be appropriately extended), then observe the digestion of cells under a microscope. If most of the cells become round and fall off, quickly take them back to the operating table, lightly tap the culture bottle a few times, and add3-4mlcontain10%FBSUse the culture medium to terminate digestion.

3. Gently mix and suck out, then1000RPMCentrifuge under conditions3-5minDiscard the supernatant and add more1-2mLBlow the culture medium evenly. Press the cell suspension1：2The proportion is allocated to the newT25Bottle, add6-8mlAccording to the instructions, configure a new specialized culture medium to maintain the growth vitality of cells. Subsequent passages will be conducted according to the actual situation1:2~1:5The proportion is carried out.

3）Cell cryopreservation:It is recommended to receive the cells before cultivation3Freeze a batch of cell seeds for future experiments.

Shanghai Yuchun Biotechnology Co., Ltd. is a technology enterprise jointly founded by personnel with excellent backgrounds in life sciences and biotechnology. The company focuses on primary cells, tumor cells, and cell culture related reagent products, providing technical support and services for universities, food, medicine, biological research, and other fields.

Since its establishment, the company has provided customized cell products and complete cell culture solutions to customers through continuous technological innovation and product research and development. Through active exploration, research and development, deepening and enriching of products, there are currently more than 200 types of basic culture medium products, more than 300 cell lines, and nearly 600 types of primary cells. We always take "developing science and technology, caring for public life and health" as our driving force. Through technological innovation, we extensively attract high-tech talents, continuously improve our product system and enrich our product chain, actively expand our market and application areas, and establish a competitive "Yuchun" brand in the market.

Precautions

1. For your safety and health, please wear lab coats and disposable gloves and masks when operating;

2. To maintain the excellent performance of this product, please be sure to store it according to the recommended storage conditions;

3. This product is only for scientific research or further production use and cannot be used for clinical diagnosis or treatment.

RC+LUC+GFP human B lymphoma cells+LUC+GFP