SGD-IV fully automatic reducing sugar analyzer

SGD­IVFully automatic reducing sugar analyzer

1、 Overview

There are many methods for determining reducing sugars, including chromatography, colorimetry, polarimetry, etc. Due to certain requirements for sample color, turbidity, composition, etc., these methods are subject to many limitations in practical applications. At present, the food fermentation industry mostly uses manual titration with Fehling reagent to determine reducing sugars. The Fei Lin reagent manual titration method is the national standard method for the determination of reducing sugars in food in China, and it is also the standard method used by the Sugar Analysis Methods Committee. The reducing sugar determination technology has been developed70Years of history, well-known to a wide range of users. This method is a manual operation with a simple process, and can accurately measure according to strict requirements. However, this manual method is susceptible to interference from many factors during the actual measurement process, such as sample amount, water volume, reagent concentration, especially manual shaking speed and force, titration speed, heating time, evaporation rate, and endpoint determination of colored sample titration, which seriously affect the accuracy of the measurement. However, different operators have different techniques for controlling these conditions, resulting in significant measurement errors and causing a lot of trouble for production process control and product quality inspection, seriously affecting the improvement of high-level control technology in the production process.

In order to adapt to the rapid development of production control technology and process detection technology in China's food fermentation industry, the Biology Center of Shandong Academy of Sciences has developed a fully automatic reducing sugar analyzer based on the principle of Fehling reagent determination. The instrument adopts complementary color micro automatic thermal titration technology, and various titration conditions are controlled by a microcomputer. The operator only needs to inject a small amount of sample into the reaction tank with an injector to automatically complete the measurement process, and automatically display and print the results. The operation is simple and easy to use, which can minimize human errors and improve the speed and accuracy of the measurement. This technology is a gap in the research of analytical instruments both domestically and internationally,Belonging to *, has obtained national inventionThe application of this technology is of great significance for improving the production and testing technology level of China's food fermentation industry and promoting technological progress of enterprises.

2、 Basic principles

Fully automatic reducing sugar analyzer (SGD-IV type)It is designed based on the principle of Fehling reagent determination, and its principle is consistent with current national standards; Fei Lin reagent is an oxidant composed of solution A and solution B. When measuring, a certain amount of A and B solution is mixed to first form copper hydroxide, and then form potassium copper tartrate complex. Methylene blue, as a titration endpoint indicator, appears blue in oxidizing solution and colorless after reduction. When using standard reducing sugar droplets, the reducing sugar first reduces copper until copper is completely reduced, and then reduces methylene blue to colorless, which is the endpoint of titration.

During the titration process, the color of the solution gradually changes: blue → dark blue → light blue → purple red → light purple red → suddenly changes to colorless and transparent at the endpoint.

Using a photoelectric conversion device to detect changes in transmittance during titration process(See figure)1）According to the voltage change curve, the instrument control system automatically records, samples, and determines the titration endpoint; The control system automatically calculates the reducing sugar content in the sample based on the standard amount of reducing sugar consumed at the end of titration.

Reason for recommendation:

1. Reagent pump, titration pump automatic exhaust bubble; 2. More convenient to operate; 3. Optimization design of precision components; 4. Shorten the time to within 3 minutes;

5. Fast measurement speed; 6. Strong anti-interference ability; 7. Not affected by color and turbidity in the sample; 8. Suitable for detecting reducing sugars in various samples.

Application fields:

Microbial fermentation:

Microbial fermentation of glutamic acid, lysine, citric acid, xanthan gum, alcohol, beer, etc.

Pharmaceuticals:

Penicillin, streptomycin, cephalosporin and other antibiotics.

Biochemical and starch sugar products:

Starch, glucose, maltose, isomaltose, oligosaccharides, fructose syrup, high maltose syrup, dextrin, sodium gluconate, etc.

Food and Beverage:

Wine, dried fruits, fruit juice, honey, pastries, and various beverages. Potatoes and their products, fruits and vegetables, and other agricultural products

Others:

Tobacco, Chinese herbal medicine, and related teaching, research, etc

Main performance indicators:

Power supply: 220V 50Hz

Host size: 421 × 342 × 230mm (L × W × H)

Weight: 12Kg

Error: ≤ 1%

Linear range: 0.01-1.00% (g/ml as glucose)

Measurement cycle: ≤ 3 minutes

Address: Institute of Biology, Shandong Academy of Sciences, No. 28789 Jingshi East Road, Licheng District, Jinan City, Shandong Province