Rotaphor 6.0 pulsed field electrophoresis in Jena, Germany

pulsed-field gel electrophoresisRotaphor 6.0
Pulsed field electrophoresis（PFGE，Pulsed Field Gel Electrophoresis）It is a method of separating large moleculesDNAThe method. Based on this technologyBiometraDeveloped a patented rotating pulse field electrophoresis system——Rotaphor 6.0System.
Rotaphor 6.0The principle of
Rotaphor 6.0Using patented rotating pulse field（ROFE，Rotating Filed Electrophoresis）, as shown in the picture1The electrode shown is placed on the rotor and driven by the rotor to rotate at any angle, generating a uniform repetitive electric field (as shown in the figure)2）. In pulsed field gel electrophoresis, the electric field is constantly changing in two directions (with a certain angle, rather than the opposite two directions). due toDNAMolecules carry negative charges and move towards the positive electrode. The relatively small molecules can quickly change the direction of movement after the electric field conversion, while the larger molecules are more difficult to turn in gel. Therefore, small molecules move forward faster than large molecules, ultimately achieving the goal of separation.
Rotaphor 6.0characteristic

·The patented rotating pulse field, with a unique and innovative pulse field design, is driven by a rotor to rotate the electrode at any angle, generating a uniform repetitive electric field; Large separation range, capable of separating up to8MbofDNAFragment; High resolution, easy to distinguish between linear and circularDNARegarding the large circular shapeDNABetter separation effect;

·Flexible functionality and applicabilityCHEF、FIGE、PAGEandROFEWaiting for large moleculesDNASeparation method;

·Buffer control system,RotaphorThe built-in buffer circulation pump in the electrophoresis cell is connected to an external refrigeration regulator, which precisely controls and monitors the temperature of the buffer solution through software to ensure constant temperature and ion balance during electrophoresis

·Easy to operate, unique software control, easy to operate, unlimited memory space, and the ability to establish one's own database; In addition to custom programs, the software comes with built-in features17A preset program for separating various fragment ranges, accompanied by a reference image

Rotaphor 6.0Application fields of pulsed field electrophoresis

·Identification of biological and microbial lineages; Research on Molecular Epidemiology

·Research on genomic libraries or large fragment plasmids; Localization of disease genes

·Mapping of genetic physics,RELPAnalysis andDNAfingerprint analysis

·Programmed death research,DNAResearch on Damage and Repair

·In addition to custom programs, the software comes with built-in features17Separate various fragments

Rotaphor 6.0Parameters for
Gel Room

·cause14The rotating electrode composed of platinum electrodes can rotate freely

·Angle setting range:95-160Degree, step value1linear measure

·Built in buffer circulation pump

·Internal cooling circuit, connected to external temperature controller

·Temperature sensitive probe

·at most2.4LBuffer

·Acrylic transparent glass safety cover (automatically cuts off power when the cover is lifted)

·Size:35 x 47 x 25 cm（W x D x H）

Rubber plate

·Adjustable horizontal support angle

· 20 x 20 cmGel size

·maximum18cmElectrophoretic distance

· 18Tooth comb, with a glue thickness of5mmMaximum sample size60μl; Optional25/40/50The comb of teeth,18Hole (for liquid samples)

·Rubber frame

controller

·Personal computer, operating systemWin XP，256MMemory,40GHard drive,40XCD drive

· RotaphorElectrophoretic Cell Control Interface

·pre-installRotaphor 6.0software

Rotaphor 6.0software

· 17Group optimization program, can be used for separation100bp-6,000kbofDNAsample

·The electrophoresis program can be classified according to the type of separated sample

·Very convenient to adjust various parameters (electrode angle, voltage, pulse time)

·The voltage range of the program used for electrophoresis0-225V（0-8.5V/cm）

·Accurate control of electrophoresis buffer temperature（5-22℃）

·For each electrophoresis program, there will be corresponding actual electrophoresis result photos displayed

·User grouping and password control

·Comprehensive online assistance

·user's manual

power supply

·Voltage:0-300V

·electric current:0-450mA

·Size:52mm x 222mm x 202mm（H x W x D）

·Weight:1.7 kg