Recombinant Human IL15

高活性Recombinant Human IL15

In 1994, scientists discovered interleukin-15 (IL-15) in their research. It is mainly produced by monocytes and macrophages, and IL-15 mRNA is expressed in various tissues of the human body such as the heart, lungs, kidneys, placenta, and muscles. The IL-15 gene is located on human chromosome 4q31 and consists of 9 exons and 8 introns, with 4 exons encoding mature proteins with a molecular weight of approximately 14-15 kDa. Recombinant human IL-15 is prepared through biotechnology and has a structure and function similar to natural IL-15.

biological function

1. Immune cell regulation:IL-15 can be regarded as an "agonist" of the immune system. On the one hand, it can stimulate the expression of anti apoptotic protein Bcl-2, which helps to improve the survival rate of NK cells and CD8+T cells, allowing these immune cells to function for a longer period of time. On the other hand, it can also promote the expression of Ki67 protein, which is closely related to cell proliferation, and accelerate the division and increase the number of NK cells and CD8+T cells. NK cells and CD8+T cells, as important cytotoxic cells in the immune system, can significantly enhance their immune strike ability against tumor cells by increasing their quantity and activity. For example, in the tumor microenvironment, IL-15 can promote the proliferation and high activity of NK cells and CD8+T cells, forming a powerful attacking posture against tumor cells.

2. Maintenance of memory T cells:In the tumor microenvironment, IL-15 plays an important role in maintaining the steady-state survival, proliferation activation, and maintenance of effector cytotoxic T lymphocytes (CTLs) of memory T cells. The presence of memory T cells can enable the immune system to quickly respond to tumor recurrence. When tumor cells reappear, memory T cells can quickly recognize and initiate an immune response, effectively suppressing tumor development.

3. Immunotherapy enhancement:Research has shown that IL-15 can enhance antibody dependent cell-mediated cytotoxicity against NK cells, thereby enhancing the efficacy of immunotherapy. Adding IL-15 to some immunotherapy regimens can significantly improve treatment efficacy.

application field

1. Tumor immunotherapy: IL-15 shows great potential in tumor immunotherapy. Both in vivo and in vitro experiments have confirmed that it can induce the activity of CTL cells and LAK cells, thereby demonstrating anti-tumor activity. For example, studies have found that IL-15 can induce LAK cell activity in lymphocytes of relatively stable melanoma patients, and has a killing effect on intrinsic melanoma cells.

2. Treatment of chronic inflammatory diseases: Taking rheumatoid arthritis as an example, the ability of IL-15 to recruit and activate T lymphocytes through chemotaxis has been confirmed. This indicates that IL-15 can play a biological role in chronic inflammatory diseases. Currently, in the treatment of rheumatoid arthritis, direct targeting of T cells has been proven to improve the disease condition. Therefore, IL-15 may become an important target for the future treatment of chronic inflammatory diseases such as rheumatoid arthritis.

3. Anti parasitic infection: IL-15 can play an immune enhancing role in combating worm and protozoan infections, which helps the human body fight against parasitic infections.

Serruicheng provides highly active recombinant human interleukin-15 (Recombinant Human IL-15)

The high activity recombinant human IL-15 provided by Serruicheng is prepared, inspected, and packaged in a clean room that meets GMP standards.

Purity: purity>95% as detected by SDS-PAGE electrophoresis;

Biological activity: ED50 less than 1 ng/ml was determined by dose-dependent proliferation assay using CTLL-2 cells;

Endotoxin: less than 1 EU/μ g;

The recombinant human IL-15 product developed by Serruicheng has been widely used in cell culture related research and industrial production, and has gained recognition from a large number of customers. Whether it is in-depth immune cell research conducted by scientific research institutions or industrial production carried out by biopharmaceutical companies, the highly active recombinant human IL-15 product from Serruicheng can provide strong support and help promote the development and innovation of related fields.