Real time monitoring system for food hygiene indicator bacteria and pathogenic bacteria

Foodborne pathogen contamination is a threat to food safety, however, traditional microbiological detection techniques cannot accurately identify and diagnose pathogens causing food contamination;

DW-1000 typeReal time monitoring system for food hygiene indicator bacteria and pathogenic bacteriaBy using a unique multiple nested thermal cycle PCR amplification instrument and a fully automatic nucleic acid electrophoresis analyzer, we provide a complete molecular typing detection scheme for foodborne pathogens and a series of multiple foodborne pathogen nucleic acid detection kits. Combined with a molecular chromatography card for rapid determination results, we provide an ideal technical platform for disease control institutions to identify and diagnose microbial contaminants.

Application of the system in nucleic acid detection of multiple pathogenic microorganisms

1. The nucleic acid detection system for multiple foodborne pathogens is based on the principle of multiplex PCR

Using a unique multi nested thermal cycle PCR amplification instrument (combining semiconductor rapid thermal cycle module and innovative multi nested cycle temperature control technology) and a fully automatic capillary electrophoresis analyzer, we provide targeted analysis for foodborne pathogensA complete molecular biology detection plan, including the entire process of sample processing, multiplex PCR amplification, and fully automated electrophoresis analysis of multiplex PCR products.

2. System nucleic acid detection simplifies traditional electrophoresis detection into three steps: sample loading, running, and data analysis

Using a pen style pre installed clamp, the sample is automatically loaded onto the capillary electrophoresis platform on a 96 well plate;96 well plate can be used for direct sample loading, or ordinary PCR tubes, 8-tube, and 12 tube can be used; Using pre installed pen clips, each experiment can be automatically completed.The minimum sample volume is only 1 µ l (with a sample volume of 0.1ul), ensuring detection even with low microbial contamination.Using pre installed card clips, plug and play, the instrument automatically cleans the capillary electrophoresis channel without the need for manual cleaning.

One step operation can automatically obtain digital qualitative and quantitative results, presented in gel or peak graphs

Standardized digital results：The system is equipped with professional analysis software for automatic processing, analysis, and collectionPCR product data; You can flexibly choose to present the results using electrophoresis peak or gel chart formats.

The sensitivity of PCR product analysis is 0.005 ng/ul,The resolution of PCR products can reach 2bp,The content of nucleic acid stain in gel is extremely low, and it is sealed and filled, so it does not pollute operators and experimental environment.

Supporting DW-1000 modelReal time monitoring system for food hygiene indicator bacteria and pathogenic bacteriaNucleic acid product analysis and comparison software:Realize intelligent capillary electrophoresis band recognition and specialized multiplex PCR detection result determination, while storing raw result data in a database to generate detection reports.

3. Use the following types of foodborne pathogen detection reagents in combination, and be compatible with analyzing various multiplex PCR reagents in the industry:

14 common foodborne pathogenic bacteria multiplex PCR detection reagents: For the 14 fold detection, a single PCR reaction is used to screen for Salmonella, Shigella, Vibrio parahaemolyticus, Staphylococcus aureus, Escherichia coli, etc O157、 Single addition of Listeria monocytogenes, Bacillus cereus, Yersinia enterocolitica, Escherichia coli, Vibrio cholerae, Campylobacter coli, Campylobacter jejuni, and Aeromonas hydrophila to meet the needs of emergency testing.

10 heavy detection reagents: multiple PCR reaction screening was performed using A+B strategy (i.e. one sample, two PCR reactions) to screen Salmonella, Vibrio, Shigella, Clostridium difficile (Cytotoxin B), Campylobacter jejuni, pestis pestis, Aeromonas, Escherichia coli O157: H7, Shiga toxin producing Escherichia coli (VTEC), etc.

Five types of multiple PCR detection reagents for diarrhea causing Escherichia coli: a 12 fold detection reagent with 12 pairs of primers to detect 11 virulence genes and 1 species identification gene, accurately typing EHEC, ETEC, EIEC, EPEC, and EAEC pathogenic types, and also screening and suggesting new strains of Escherichia coli pathogenic bacteria.

Quickly identify four types of Shigella, comprehensively covering different serotypes of Shigella from Songnei and Fusarium;

Quickly identify Vibrio parahaemolyticus and its characteristic virulence genes;

Rapid identification of Escherichia coli O157: H7 and its characteristic virulence gene profile;

Solution for rapid identification of Yersinia enterocolitis and its specific virulence gene profile.

Other applications of multiplex nucleic acid amplification testing can be developed according to customer needs and technical support can be provided.

Instrument configuration

DW-1000 System Configuration

1. The system includes products and services:

2. DW-1000 Multi nested Hot Cycle PCR Amplifier (optional);

3. Qsep100 fully automatic capillary nucleic acid electrophoresis system and DW-1000 nucleic acid product analysis and result comparison software;

4. Equipped with multiple microbial multiplex PCR amplification detection kits;

5. Complete technical services for processing foodborne microbial nucleic acid samples and microbial genotyping.