Rat Osteopontin (OPN) ELISA Kit

Rat Osteopontin (OPN) ELISA KitDetailed introduction:

[Specification]: 48T/96T

Brand: Ruifan

: 450nm

: 2-8 ° C, six months

: For scientific research purposes only

[Purpose of Detection]: Used for the determination of serum, plasma, pleural and peritoneal fluid and other related liquid specimens

[Features]: High sensitivity, strong specificity, good repeatability, stable reagents, easy storage, simple operation

The species supplied by Ruifan include: humans, chickens, ducks, fish, horses, rabbits, pigs, plants, etc. ELISA kits, etc

Rat Osteopontin (OPN) ELISA KitSpecimen processing:

1. Our company is only responsible for the reagent kit itself and is not responsible for the sample consumption caused by the use of the kit. Users are advised to fully consider the possible usage of the sample and reserve sufficient samples before use.

2. Before the experiment, the sample content should be predicted. If the sample concentration is too high, the sample should be diluted to meet the detection range of the kit, and then multiplied by the corresponding dilution factor during calculation. The specimen was diluted with 0.01mol/L PBS (pH=7.0-7.2).

If the tested sample is not included in the samples listed in the instruction manual, it is recommended to conduct a pre experiment to verify its effectiveness and pay attention to retaining the sample.

4. Tissue homogenates or cell extracts prepared using chemical lysates may result in biased ELISA experimental results due to the introduction of certain chemicals.

5. If the sample is a cell culture supernatant, there may be many interfering factors such as cell status, cell quantity, sampling time, etc., so there may be situations where it cannot be detected.

6. Some natural or recombinant proteins, including prokaryotic and eukaryotic recombinant proteins, may not be detected due to mismatches with the detection and capture antibodies used in this product.

7. It is recommended to use fresh samples, as prolonged storage may lead to protein degradation or denaturation, resulting in biased experimental results.

Operation steps:

1. Sample addition: Set up blank wells (blank control wells without sample or enzyme-linked immunosorbent assay, all other steps are the same), standard wells, and test sample wells. Add 50 μ l of the standard sample to the enzyme-linked immunosorbent assay (ELISA) coated plate. First, add 40 μ l of sample diluent to the well of the test sample, and then add 10 μ l of the test sample (with a final dilution of 5 times). Add the sample to the bottom of the enzyme-linked immunosorbent assay plate well, avoiding touching the well wall as much as possible, and gently shake and mix well.

2. Incubation: Cover the plate with a sealing film and incubate at 37 ℃ for 30 minutes.

3. Preparation: Dilute 30 times the concentrated washing solution with distilled water and set aside for later use.

4. Washing: Carefully remove the sealing film, discard the liquid, shake dry, fill each hole with detergent, let it stand for 30 seconds, then discard. Repeat this process 5 times and pat dry.

5. Enzyme addition: Add 50 μ l of enzyme labeled reagent to each well, except for blank wells.

6. Color development: Add 50 μ l of color developing agent A50 to each well, then add 50 μ l of color developing agent B50, gently shake and mix, and develop color at 37 ℃ in the dark for 10 minutes.

7. Termination: Add 50 μ l of termination solution to each well to terminate the reaction (at this point, the blue color turns yellow).

8. Measurement: Measure the absorbance (OD value) of each well in sequence using a blank air conditioner with a wavelength of 450nm. The measurement should be conducted within 15 minutes after adding the termination solution.

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Answer to the question:

If the experiment effect is not good, please take photos of the color development results in time, keep the Flat noodles used and unused reagents, and keep them properly, then contact our technical support to solve the problem for you. You can also refer to the following materials: