Rat N-terminal pro brain natriuretic peptide ELISA kit in stock

Rat N-terminal pro brain natriuretic peptide ELISA kit in stockDetailed introduction:

[Specification]: 48T/96T

Brand: Ruifan

: 450nm

: 2-8 ° C, six months

: For scientific research purposes only

[Purpose of Detection]: Used for the determination of serum, plasma, pleural and peritoneal fluid and other related liquid specimens

[Features]: High sensitivity, strong specificity, good repeatability, stable reagents, easy storage, and simple operation.

The species supplied by Ruifan include: humans, chickens, ducks, fish, horses, rabbits, pigs, plants, etc. ELISA kits, etc

High quality spot direct sales of ELISA kit, free testing, welcome to call for product manual

Validity period: 6 months

Expected application: ELISA method for quantitative determination of content in serum, plasma, cell culture supernatant or other related biological fluids.

Storage temperature: 2-8 ℃ Low temperature storage

Purpose: Scientific research in fields such as scientific experiments, not for clinical diagnosis

Species: humans, rats, mice, rabbits, pigs, dogs, monkeys, horses, cows, sheep, chickens, ducks, fish, etc

Transportation method: Spot supply, usually express delivery, arriving the next day in Jiangsu, Zhejiang, and Shanghai, and taking three to five days to arrive in other and remote areas

Rat N-terminal pro brain natriuretic peptide ELISA kit in stock：

1）Before use, mix all reagents thoroughly. Do not make the liquid produce a lot of foam, so as not to add a lot of bubbles when adding samples, which will cause errors in adding samples.

2）Determine the number of Flat noodles required according to the number of samples to be tested plus the number of standards. It is recommended to make duplicate holes for each standard and blank hole. Each sample is determined according to its own quantity, and those that can be reused should be reused as much as possible. Sample dilution solution for specimens1：1Add after dilution50ulInside the reaction well.

3）Add diluted standard samples50ulAdd the test sample to the reaction well50ulInside the reaction well. Join now50ulThe labeled antibody. Cover the membrane plate, gently shake and mix well,37℃ incubation1Hour.

4）Shake off the liquid in the hole, fill each hole with detergent, and shake30In seconds, shake off the detergent and pat dry with absorbent paper. Repeat this operation3 Next time. If washing with a washing machine, increase the number of washes by one.

5）Add to each hole80ulAffinity Chain Enzyme-HRPGently shake and mix well,37℃ incubation30minute

6）Shake off the liquid in the hole, fill each hole with detergent, and shake30In seconds, shake off the detergent and pat dry with absorbent paper. Repeat this operation3Next time. If washing with a washing machine, increase the number of washes by one.

7）Add substrate to each wellATheBeach50ulGently shake and mix well,37℃ incubation10minute Avoid exposure to light.

8）Remove the enzyme-linked immunosorbent assay (ELISA) plate and quickly add it50ulTermination solution, the results should be measured immediately after adding the termination solution.

9）In450nmMeasure the wavelength of each holeODValue.

6The results above the standard sample are non-linear, and accurate results cannot be obtained based on this standard curve.

1. Sensitivity:***The small detection concentration is less than1Standard product number. The linearity of dilution. Correlation coefficient between sample linear regression and expected concentrationRvalue0.990.

2. Specificity: Does not react with other cytokines.

3. Repeatability: The coefficient of variation within and between boards is less than10%.