Beckman Coulter MoFlo XDP ultrafast flow cytometry cell sorting system | MoFlo XDPRapid flow cytometry cell sorting systemIt can identify, classify, quantify, and isolate cells in complex samples, and can simultaneously perform ultra high speed sorting and purification, high-throughput monoclonal sorting, or cell chip preparation on one to four specific cells in a single operation. The sorted cells can be directly used for cultivation, transplantation, nucleic acid extraction, single-cell PCR amplification, or in situ hybridization, and can further be used for research on cell genes, proteins, functional levels, and differentiation between different cells. The proportion of discarded sample cells in hardware is less than 5%, ensuring a high recovery rate of target cells in the sample, especially suitable for research on extremely low content cells such as stem cells.
MoFlo XDPCan be used to detect and sort various eukaryotic and prokaryotic cells, plant cells, microorganisms, plankton, etc. Specially designed for researchers who require high yields, biosafety, and convenient software analysis systems. | | | | | | | |
| |
MoFlo: a sorting brand highly respected by scientists worldwide
MoFlo XDP adheres to the legendary bloodline and continues to write brilliance
As the world's strongest flow cytometry system today, MoFlozui has long established the gold standard for flow cytometry, making outstanding contributions to promoting the application of cell sorting in the scientific community and enjoying a high reputation among scientists worldwide. As the generation of this system, MoFlo XDP has once again established the gold standard for flow sorting in the 21st century, leading the new trend of flow sorting.
MoFlo XDP: Utilizing advanced electronic systems and automation software to create a powerful signal computing heart, achieving the fastest and fastest results
MoFlo XDP fully utilizes its powerful electronic system to meet the requirements of high speed, high purity, high recovery rate, and high accuracy for flow separation. The application of multiple ADC with high-speed computing capabilities greatly increases the sampling density of fluorescence signals. Its actual cell sampling speed can far exceed 100000/s, without missing any cell signal. It is equipped with a truly automatic sorting setting and stabilization system, making sorting simple and easy to implement while ensuring accuracy and reliability. In addition, MoFlo XDP has a dedicated high-performance server to process and convert received signals, providing powerful hardware support for ultra high speeds. MoFlo XDP starts from the heart and spares no effort to create the most high-end sorting streaming platform in the industry, becoming the eternal pursuit of other sorting streaming platforms.
MoFlo XDP: Unique sorting design that truly cares about cells
MoFlo XDP: A Platform for Rare Cells and High End Applications
Statistically correctScientific experimental results require good accuracy and reproducibility. Accuracy can be defined as the signal obtained from the flow equation being specific and correct, while repeatability is reflected in statistics, requiring the coefficient of variation of the data results from parallel experiments to be controlled within a certain range.
This table shows the required total number of cells to be obtained and preserved for different proportions of rare cells with varying coefficients of variation.
One Rare cell applications
After labeling CFSE, it was transplanted into recipient mice through the tail vein, and spleen cells were extracted from the recipient mice for transplantation cell detection. A total of 22.93 million cells were tested, and it was found that the transplanted cells had undergone 6 generations of proliferation. R7, R8, and R9 are the 5th, 4th, and 3rd generation transplanted cells, with contents of about one ten thousandth each.
CD133 positive cells with a content of about 1/100000 were sorted and directly injected subcutaneously into nude mice for tumor formation experiments.
2、 Low content, continuous expression of cells for high-speed four-way sorting simultaneously
3、 Tumor stem cell detection
Tumor stem cells typically lack specific markers and have low levels, making them difficult to detect. Compared with ordinary tumor cells, tumor stem cells contain the ABC protein family with pumping function on their cell membrane, which is a decisive factor in tumor drug resistance and recurrence. Therefore, research on tumor stem cells is of great significance.
By utilizing the pumping function of its cell membrane and using the small molecule reactive dye Hoechst33342 for balanced staining of tumor samples, side population cells (SP) with less staining due to Hoechst33342 pumping can be detected, which are known as tumor stem cells. Using the pump protein inhibitor Verapami for simultaneous staining, it can be observed that the proportion of lateral group cells significantly decreases or completely disappears, confirming the location of SP cells. Other stem cells, such as mesenchymal stem cells, hematopoietic stem cells, neural stem cells, various adult stem cells, etc., can be screened using this method. Sorting out SP cell populations and non SP cell populations can be used to study major life science topics such as gene and protein expression differences, pathogenic mechanisms, and drug screening.
4、 Chromosome/Sperm Analysis and Sorting
The high-precision signal resolution capability of MoFlo/MoFlo XDP (stable liquid flow, low laser/electronic system/PMT noise/fast processing speed) and the high activity harvesting of cells (good system cleanliness) are fully demonstrated in chromosome and sperm sorting. The artificial insemination of cow sperm for the production of small dairy cows has great economic benefits in animal husbandry. MoFlo/MoFlo XDP is currently the most suitable sorting flow cytometer for this application. There are 30 MoFlo machines operating day and night for commercial sorting in China.
The difference in DNA content between different chromosomes is very small, and ordinary DNA dyes cannot accurately distinguish 23 pairs of chromosomes. Chromosome DNA was stained using HOECHST 33258 excited by high-power 355nm ultraviolet laser and CA3 excited by high-power 458nm laser, respectively. Different chromosomes are separated due to their different ratios of G-C/A-T base pairs. Selecting any chromosome and setting a gate allows for high-purity sorting. Chromosome analysis sorting can be applied in high-precision FISH, chromosome translocation, gene localization, chromosome sequence determination, chromosome related protein analysis, etc. |
Features of MoFlo XDP:
By utilizing advanced electronic systems and automation software, we aim to create a powerful electronic processing system and achieve the fastest signal acquisition rate.
The gold standard for air excitation and flow sorting.
Four way sorting, multiple sorting modes, can be collected using 6-1536 well plates.
Multiple nozzle specifications and unique design ensure cell viability, truly caring about cells.
A platform for rare cells and high-end applications, a powerful assistant for stem cell research. |
Technical parameters for cell analysis of MoFlo XDP superfluid sorting system:
Acquisition speed capability:>100000 cells/second (under any amount of fluorescence and compensation).
Sorting speed capability:>70000 cells/second (under any amount of fluorescence and compensation).
Sorting purity:>99% (at any speed).
Sorting pathway: 4 channels, capable of receiving 0.2-50ml centrifuge tubes.
Clone sorting: 6-1536 microplate, glass slide or any matrix of customer specifications, the same well plate and glass slide can be used to sort different cells in multiple ways.
Recovery rate: 100% Poisson distribution.
Fluorescence sensitivity: FITC<100 MESF, PE<50 MESF.
Droplet oscillation frequency: 200kHz (up to 200000 droplets per second).
Signal Processor (ADC) processing speed: 100MHz (0.01 µ sec sampling frequency).
Signal Processor (ADC): 2.
Signal pulse accuracy: 32-bit (2)32,I.e. route 4294967296).
Fluorescence linear range: 5 logarithmic thresholds.
Single acquisition and storage of cellular information: 1 billion cells.
Signal quantity: 2 scattered lights, up to 21 fluorescent lights.
Signal type: Each fluorescence can simultaneously collect 5 types of signals (Height, Width, Area, Log Height, Log Area).
Laser excitation circuit: 3, each can achieve 3 laser collinearity (optional laser collinear).
Laser configuration (optional): 488nm, 200mW solid; 405nm, 50mW solid;
635nm, 25mW diode; 355nm, 100mW solid UV;
640nm, 100mW solid; 561nm, 100mW solid;
532nm, 200mW solid; 460nm, 500mW solid;
Various specifications of Coherent water-cooled lasers and other customer lasers.
Filter system: Standard package and Hoechst, DAPI, INDO, APC/APC Cy7, optional BSLI or II level safety cabinet.
Nozzle types: 8 types, 50 µ m-200 µ m.
The minimum resolution particle size is less than 0.2 µ m.
System pressure: 4-100 PSI.
Software: Summit Software version 5.2 or above, open for installation.
Compensation method: 21 X 21 full matrix automatic compensation, offline compensation, dual zoom coordinate display.
Operating System: MicrosoftTMWindowsTMXP Professional。
Work platforms: NI servers and Dell high-performance workstations.
The main purpose of MoFlo XDP is to identify, classify, quantify, and isolate cells in complex samples. It can simultaneously perform ultra high speed sorting and purification, high-throughput cloning and distribution, or cell chip preparation on one to four specific cells in a single operation. The sorted cells can be directly used for cultivation, transplantation, nucleic acid extraction, single-cell PCR amplification, or in situ hybridization, and can further be used for research on cell genes, proteins, functional levels, and differentiation between different cells. Suitable for various eukaryotic and prokaryotic cells, plant cells, microorganisms, plankton, etc. |
| | | |
