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Beckman Coulter Trading (China) Co., Ltd
BioLector XT Next Generation High Throughput Microbioreactor
NegotiableUpdate on 02/21
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Overview
The BioLector XT next-generation high-throughput microbioreactor is a desktop device used for high-throughput screening of microbial cultures, which can monitor common culture parameters online, such as biomass, pH value, dissolved oxygen (DO), and fluorescence intensity of various fluorescent molecules or proteins. In order to achieve high throughput, we cultured on SBS/LAS standardized microplates (48 wells per plate), allowing for the simultaneous processing of up to 48 samples in a BioLector XT microreactor.
Product Details
BioLector XTNew generation high-throughput microreactors
can beBioLector XTSuccessfully conducted anaerobic biological cultivation experiments in a micro bioreactor. Simultaneously conducting small-scale cultivation by combining microfluidic chip technology and gas processorspHControl, supplement materials, and directly fill nitrogen gas.
system
BioLector XTThe new generation high-throughput microbioreactor is a desktop device used for high-throughput screening of microbial culture, which can monitor common culture parameters online, such as biomasspHValue, dissolved oxygen（DO）And the fluorescence intensity of various fluorescent molecules or proteins. In order to achieve high throughput, we areSBS/SLASStandardized microplate (per plate)48Cultivate on a single well, so that it can be grown on one machineBioLector XTSimultaneous processing of up to48A sample.
automation
BioLector XTOne of the main advantages of a micro bioreactor gas processor is its ability to operate on microporous plates（MTP）Directly adding nitrogen (such as100%concentrationN2）Simultaneously replenishing and controlling materials during the processpHThe nitrogen flow rate can be5–50 mL/minAdjust within the range
consumables
All fed batch cultures are conducted inNextGen-MicrofluidicCircular hole plate (Figure)2）Go ahead.AFill in luggage1,900µLGlucose supplement solution,BFill in luggage1,900µL pHtuneSaving agent. inIn BioLector software, if it is an aqueous solution (3M NaOH),Adjust the pump volume to0.30 µ L, viscous feed liquid (500g/L grape)Sugar is adjusted to0.16 µ L. In all feeding batch cultivation experiments, according to timeTrigger the replenishment operation, set the replenishment speed to a constant value4 µ L/h. PH control is set topH=6.0InNextGen-MicrofluidicCultivation on a circular hole plateDuring the process, by usingBioLector XT Micro Bioreactor Gas Processor ObtainedThe gas processor is installed in a breathable sterile sealing membrane in an anaerobic environment（F-GPRSMF32-1) on sealed MTP.
Measurement schematic
BioLector XTThe new generation high-throughput microbioreactor combined with its anaerobic gas processor has been technically and biologically validated for cultivating probiotics such as Lactobacillus and Bifidobacterium bifidum. Microfluidic chip technology combined with gas processors can be used simultaneously in small-scale cultivation systemspHControl, feed, and direct nitrogen filling. This system is suitable for anaerobic bacterial cultivation.
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