RXFP2 K658E CHO

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

RXFP2 [K658E] CHO

Product Details

RXFP2 [K658E] CHO

I. Background
The RXFP (Relaxin Family Peptide Receptor) family is a type of G protein coupled receptor, consisting of four members: RXFP1, RXFP2, RXFP3, and RXFP4. RXFP2， Also known as LGR8 or GREAT, its protein structure has seven transmembrane domains, an extracellular rich repeat sequence, and a low-density lipoprotein A-class module. Its main physiological ligand is insulin-like peptide 3 (INSL3). During fetal development, INSL3 binds to RXFP2 to activate downstream signaling pathways (such as increasing intracellular cAMP levels), driving the transabdominal descent of the testes; Functional failure can lead to bilateral cryptorchidism. After entering puberty, the expression level of RXFP2 increases with the increase of testosterone and directly participates in spermatogenesis and maturation, especially during the differentiation stage of round sperm cells into mature sperm. Impaired function of this receptor can lead to spermatogenic arrest and non obstructive azoospermia. In addition, RXFP2 may also be involved in physiological processes such as immune regulation and bone metabolism.

II. Description
The RXFP2 [K658E] CHO cell drug target model effectively simulates the signal transduction process of RXFP2 [K658E] in vivo, as shown in the following figure. Figure 1. Schematic diagram of RXFP2 [K658E] CHO cell model

III. Introduction
Expressed gene:	RXFP2 [K658E]
Host Cell:	CHO
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12K+10%FBS+5 μg/ml Puromycin
Mycoplasma Status:	Negative
Storage:	Liquid nitrogen immediately upon delivery
Application(s):	Functional assay for RXFP2 [K658E] Receptor
Assay Format:	Gs

IV. Description of Host Cell Line
Organism:	Hamster
Tissue:	Ovary
Morphology:	Epithelial
Growth Properties:	Adherent

V. Representative Data Figure 2. HTRF cAMP Assay with RXFP2 [K658E] CHO(C13) in 500 μM IBMX.