RPMI 1640 cell culture medium

GB/T 12433.2-2005Inspection methods for medical infusion, blood transfusion, and injection equipment

Cell toxicity test This experiment will use the medical device extraction solution in contact with itRPMI 1640Cell culture mediumCultivate cells and evaluate the toxicity of the test substance on in vitro cells by observing their morphology, proliferation, and inhibitory effects.

RPMI 1640 cell culture medium was developed by Moore in 1967 at the Roswell Park Memorial Institute in Farrow, New York, USA. RPMI is a type of cell culture medium developed by the institute, and 1640 is the culture medium code. RPMI-1640 cell culture medium is an improved version of McCoy's 5A medium, using a bicarbonate buffer system. Unlike most mammalian cell culture media, it has a typical pH 8 formulation. RPMI 1640 cell culture medium was originally designed specifically for lymphocyte culture and has now been widely used for the cultivation of various normal cells and cancer cells, especially suspension cell culture, which is one of the most widely used culture media.

RPMI 1640 cell culture mediumUnlike other culture media, it contains the cyclic agent glutathione and high concentrations of vitamins.RPMI 1640 cell culture mediumContains biotin, vitamin B12, and PABA, which are not present in traditional culture media. In addition, the concentrations of vitamins inositol and choline are very high.

RPMI 1640 cell culture mediumDoes not contain proteins, lipids, or growth factors. Therefore, RPMI 1640 cell culture medium needs to be supplemented, commonly using 10% fetal bovine serum (FBS).

RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), therefore 5-10% C is requiredO2Maintain physiologyThe environment with pH value.