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Shanghai Yanjin Biotechnology Co., Ltd
RNA binding protein immunoprecipitation (RIP) experimental service
NegotiableUpdate on 06/12
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Overview
Provider: Shanghai Yanjin Biotechnology Service Name: RNA Binding Protein Immunoprecipitation (RIP) Experimental Service Quotation Specification: Experimental Service Price: 2000 yuan
Product Details
Validation of intracellular RNA protein binding: RIP
Experimental significance
Studying the binding of RNA and protein in cells is helpful to understand the dynamic process of the post transcriptional regulatory network, discover the regulatory targets of miRNA, understand the overall level of RNA changes and changes in the post transcriptional regulatory network such as malignant tumors and genetic diseases, and apply them to pathological diagnosis and scientific research.
Experimental principle and steps
The basic principle of RNA Binding Protein Immunoprecipitation (RIP) is to use antibodies against the target protein to capture endogenous RNA protein complexes, then separate the RNA protein complexes together through immunoprecipitation, and finally use RT-PCR RIP Seq (high-throughput)
Sequencing or RIP Chip (microarray) are used to identify the bound RNA sequence. The RIP process is shown in the following figure
Result display:
Experimental cycle and delivery standards
1. Experimental period: 1-3 months
2. Delivery standards: RIP identification result report, analysis report, experimental report (reagents, consumables, experimental steps)
Raw data, etc
Information to be confirmed
1. Target gene and protein information.
2. Do you provide antibodies
3. Sample grouping and analysis requirements
References
[1] Gagliardi M, Matarazzo M R. RIP: RNA Immunoprecipitation[J]. Methods in Molecular Biology, 2016, 1480:73.
[2] Meyer K，K? Ster T，Nolte C . et al. Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7[J] Genome Biology, 2017, 18(1):204.
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