Quantagene series fluorescence quantitative PCR system

Quantagene series fluorescence quantitative PCR system

Quantagene's entire product line uses fitting algorithms to calculate Ct values, without the need for additional reference dyes for optical correction, simplifying experimental steps while achieving excellent inter well and inter instrument repeatability: different positions, reaction volumes, and plate experiments do not affect quantitative results.

The Q225 and Q900 series adopt a recommended reaction volume of 5-10 μ l, which is particularly suitable for precise quantification under small reaction volumes. Compared with similar instruments, it saves 50% -80% of reagent usage and can obtain more accurate detection results.

The Q225 Plus series has a wide volume adaptability of 2-150 μ l, with more diverse application scenarios, and can also achieve satisfactory results in quantitative experiments of ultra large reaction systems.

In PCR reactions, temperature changes are controlled to repeatedly unwind and extend nucleic acid molecules, thereby achieving exponential amplification of the target fragment. Therefore, accurate temperature control and rapid temperature change system are the foundation and key to achieving efficient qPCR experiments. The Quantagene series qPCR instrument adopts a tightly designed thermal cycling system to achieve efficient heat conduction while significantly reducing edge effects, ensuring consistent detection accuracy at any well position.

Quantagene series fluorescence quantitative PCR system

Quantagene's entire product line adopts a static optical system and does not contain any movable parts, therefore it has better stability compared to other optical systems that include scanning or filter switching. No calibration is required during long-term use or after transportation, greatly reducing the maintenance burden on users. The Quantagene series multi-channel models feature groundbreaking Static Optical Module Array (SOMA) technology, with each optical channel containing a complete set of independent long-life LED light sources, filter groups, lens groups, and CMOS cameras. While maintaining the speed and accuracy of fluorescence signal acquisition, it greatly improves the long-term stability and channel separation of multi detection.

A. Q225 Plus can provide Ct values with significantly higher consistency than similar products. Left: Experiments were conducted using a certain A brand fluorescence quantitative PCR instrument. The same reaction solution was used in all 96 well plates, with a reaction volume of 20 μ l and a distribution of Ct values. Right: Conduct experiments using the q225Plus-400 fluorescence quantitative PCR instrument, using the same reaction solution in all 96 well plates, with a reaction volume of 20 μ l, and the distribution of Ct values.

B. Perform a continuous 2-fold gradient dilution experiment using Q225, with a reaction volume of 10 μ l and three parallel reactions for each concentration. Within the Ct value range of 4-30, q225 can be accurately quantified.

C. Use Q225 to test the difference in small fold dilution concentration, with a reaction volume of 10 μ l and 10 parallel reactions for each concentration. The five colors correspond to the amplification curves of five different concentration samples with the same template. From left to right are the original concentration (dark blue), 1.3-fold dilution (red), 1.5-fold dilution (green), 1.7-fold dilution (light blue), and 2-fold dilution (yellow). The local magnified image shows that q225 can distinguish concentration differences as low as 1.1 times.