Qualitative detection reagent for Leishmania parasite antibodies (kala azar)

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Qualitative detection reagent for Leishmania parasite antibodies (kala azar)

Guangzhou Jianlun Biotechnology Co., Ltd

Our company also provides other imported or domestically produced reagent kits:Dengue fever, malaria, influenza, Streptococcus pneumoniae, syncytial virus, mumps, Japanese encephalitis, Zika, yellow fever, chikungunya fever, Klebsiella pneumoniae, prohibited substance abuse, pneumococcal, Legionella, cosmetics testing, food safety testingWaiting for the reagent kit andJapanese bacterial typing diagnostic serum, German SiFin diagnostic serum, Danish SSI diagnostic serumWaiting for the product.

Leishmania donovani IgG diagnostic reagent

Experimental principle

Indirect enzyme-linked immunosorbent assay (ELISA). Standard samples with known LSM Ab concentration and samples with unknown concentration are added to microporous ELISA plates for detection. First, incubate LSM Ab and labeled antibodies simultaneously. After washing, add a kiss

HRP labeled with biotin. After further incubation and washing, remove unbound enzyme conjugates, then add substrates A and B, and work together with enzyme conjugates

Use it. Generate color. The depth of color is proportional to the concentration of LSM Ab in the sample.

Bring your own materials

Distilled water.

加样器： 5ul、10ul、50ul、100ul、200、500ul、1000ul。

Oscillators and magnetic stirrers, etc.

safety

Avoid direct contact with termination solution and substrates A and B. Once in contact with these liquids, please rinse with water as soon as possible.

Do not eat, drink, smoke or use cosmetics during the experiment.

Do not use your mouth to suck any ingredients from the reagent kit.

Operation precautions

Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored.

The Flat noodles not used in the experiment shall be immediately put back into the packaging bag and sealed for storage to avoid deterioration.

Other unused reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.

Use disposable suction tips to avoid cross contamination, and avoid using sample dispensers with metal parts when suctioning termination solution and substrates A and B.

Use clean plastic containers to prepare detergent. Mix all components and samples in the reagent kit thoroughly before use.

When washing the enzyme-linked immunosorbent assay (ELISA) plate, it should be thoroughly dried. Do not directly put the absorbent paper into the ELISA reaction well to absorb water.

Substrate A should evaporate and avoid opening the lid for a long time. Substrate B is sensitive to light and should be avoided from prolonged exposure to light. Avoid contact with hands, toxic. real

After the verification is completed, the OD value should be read immediately.

The order of adding reagents should be consistent to ensure that the incubation time for all reaction plate wells is the same.

Perform incubation operations according to the time, amount, and sequence indicated in the instructions.

Sample collection, processing, and preservation methods

Serum - Avoid any cellular stimulation during operation. Use test tubes without pyrogen and endotoxin. After collecting blood, centrifuge at 1000 × g for 10 minutes

Quickly and carefully separate serum and red blood cells.

Plasma - EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles.

Cell supernatant - Centrifuge at 1000 × g for 10 minutes to remove particles and polymers.

Storage - If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. Try not to use hemolysis or hyperlipidemia as much as possible

Fat blood. If there are a large number of particles in the serum, centrifuge or filter before detection. Do not thaw by heating at 37 ℃ or higher. It should be thawed at room temperature and

Ensure that the sample is uniformly filled and thawed.

Preparation of reagents

Standard: The series dilution of standard should be prepared during the experiment and cannot be stored. Shake and mix the standard before dilution.

Dilution of washing buffer (50x): Dilute 50 times with distilled water.

operating steps

Before use, mix all reagents thoroughly. Do not make the liquid produce a large amount of foam, so as to avoid adding a large amount of bubbles during sample adding, which may cause errors in sample adding

Poor.

Determine the number of Flat noodles required according to the number of samples to be tested plus the number of standards. It is recommended to make duplicate holes for each standard and blank hole. Each sample is based on its own

The quantity is determined by oneself, and those who can use double holes should try to do them as much as possible.

Add 50ul of diluted standard substance to the reaction well and 50ul of the test sample to the reaction well. Immediately add 50ul of labeled antibody.

Cover the membrane plate, gently shake and mix well, and incubate at 37 ℃ for 1 hour.

Shake off the liquid in the holes, fill each hole with detergent, shake for 30 seconds, shake off the detergent, and pat dry with absorbent paper. Repeat this operation 3 times. If using a washing machine

Wash, increase the number of washes by one.

Add 80ul of affinity chain enzyme HRP to each well, gently shake and mix well, and incubate at 37 ℃ for 30 minutes.

Shake off the liquid in the holes, fill each hole with detergent, shake for 30 seconds, shake off the detergent, and pat dry with absorbent paper. Repeat this operation 3 times. If using a washing machine

Wash, increase the number of washes by one.

Add 50ul of substrate A and B to each well, gently shake and mix well, and incubate at 37 ℃ for 10 minutes. Avoid exposure to light.

Take out the enzyme-linked immunosorbent assay (ELISA) plate and quickly add 50ul of termination solution. The results should be measured immediately after adding the termination solution.

Measure the OD values of each well at a wavelength of 450nm.

Here are some of the infectious disease products sold by our company:

ELISA kit for detecting the antigen of filarial parasites
ELISA kit for detecting the antigen of filarial parasites
Mexican Leishmania IgG kit
Leishmania donovani IgG kit
Cryptosporidium/Giardia Antigen Direct/Indirect Immunofluorescence Detection Kit
Cryptosporidium antigen direct immunofluorescence detection kit
Canine Brucella IgG ELISA kit
Pan malaria antigen (pLDH) ELISA kit
Pan malaria antigen (pLDH) ELISA kit
Plasmodium falciparum antigen (HRP2) ELISA kit
Plasmodium falciparum antigen (HRP2) ELISA kit
Entamoeba histolytica antigen ELISA kit
Jia Di worm antigen ELISA kit
Silk worm IgG ELISA kit
Dog bow roundworm IgG ELISA kit
Trypanosoma cruzi IgG ELISA kit

Qualitative detection reagent for Leishmania parasite antibodies (kala azar)

Guangzhou Jianlun Biotechnology Co., Ltd. comprehensively supplies professional testing reagents covering drug abuse, animal derived ingredients, cow and sheep milk ingredients, real and fake meat, microorganisms, biosafety, prohibited items, food safety, animal diseases, tropical infectious diseases and other fields. At the same time, it provides routine testing reagents frequently used in scientific research and daily work of disease control systems, providing a one-stop solution for the testing needs of related industries.