Proteomix ion exchange liquid chromatography column SAX-NP

Proteomix SCX-NP3（3µm）Short column for three protein mixtures (ribonuclease)ACytochromeCThe separation spectrum of lysozyme. Lysozyme in5cmThe separation efficiency on the long column has reached100,000The theoretical number of trays, such a separation effect is unprecedented in Qian.´ProteomixNon porous resin has three characteristics. Firstly, the hydrophilic layer W with nanometer thickness has been completely eliminatedPS/DVBNon specific interactions with biological samples. Secondly, the pore free particle structure minimizes the lateral diffusion of the sample and prevents its diffusion into the interior of the filler particles. Thirdly, the proprietary technology of Sai Branch Company can synthesize uniformly dense ion exchange layers. Designed with Du's ingenuityProteomix SCX-NPTheWCX-NPTheSAX-NPandWAX-NPResin can provide excellent resolution and separation efficiency for proteins, oligonucleotides, sugars, and peptides.

The smaller the particle size of the filler, the higher the separation efficiency and the faster the separation speed.ProteomixThe particle size of ion exchange resin has various specifications, ranging from1.0 µmto10 µmUnequal, it can be used for different purposes such as analysis and semi preparation. use1.7、3、5and10 µmThe spectra obtained from the separation of three proteins using weak cation exchange resins with four different particle sizes are shown in the figure7From the graph, it can be seen that the smaller the particle size, the higher the separation efficiency and resolution. For example, in lysozyme, one label isNImpurities, in use10 µmParticle sizeWCXThe column cannot be separated significantly. in use5 µmParticle sizeWCXOn the column, the baseline separation is basically achieved. When the particle size decreases to3 µmAt this time, baseline separation can be obtained. And when the particle size further decreases to1.7 µmImpurities can be completely separated into W.

ProteomixThe ion exchange resin adopts the synthesis technology of Du Chuang, a subsidiary of Sai, and the resulting non porous resin has high ion exchange capacity. The filler is rigid, spherical, and highly cross-linked polystyrene/Diethylbenzene(PS/DVB)particle The particle size of non porous fillers includes1、1.8、3、5and10 μmWaiting for several specifications.PS/DVBThe resin surface is bonded with a highly hydrophilic nano thick neutral polymer film. hydrophobicPS/DVBThe resin surface is completely covered with this hydrophilic material, thereby eliminating the need forPS/DVBThe irreversible adsorption of biomolecules ensures high separation efficiency and biological sample recovery rate. By utilizing the chemical bonding techniques available in Saifen Di, ion exchange functional groups can be densely and uniformly bonded to the surface of the hydrophilic layer, resulting in a high-capacity ion exchange layer. non-porousProteomixThe unique feature of ion exchange resin lies in its combination of high capacity and the inherent high separation efficiency of non porous resins.ProteomixIon exchange resin can be used for the separation of proteins, oligonucleotides, peptides, polysaccharides, cell lysates, nanoparticles, and nanotubes.

Product Features

· Monodisperse filler particles

· widepHScope:2-12

· Excellent separation efficiency, selectivity, and separation capability

· High voltage tolerance:10、5The3 &1.7 μmCan tolerate them separately4000, 6000, 8000 & 12000 psi

· High resolution analysis of differences among different biological species

· 1.7 & 3 μmParticle size fillers Zuj are suitable for whole protein separation and analysis in proteomics

· Suitable for peptide, carbohydrate, polysaccharide, protein, polynucleotide, cell lysate, and multidimensional chromatographic separation

Ionic exchange stationary phase with strong cation exchange（SCX）Weak cation exchange（WCX）Strong anion exchange（SAX）Weak anion exchange（WAX）Four types.Proteomix SCXTheWCXTheSAXTheWAXThey are respectively formed by chemically bonding sulfonic acid groups, carboxyl groups, quaternary ammonium groups, and tertiary amine groups in a non porous environmentPS/DVBFormed on the surface of resin hydrophilic coating.