-
E-mail
dawei.zhao@aichrom.com
-
Phone
15210979260
-
Address
No.1 Zhongguancun North Second Street, Haidian District, Beijing
Product Categories
Ningbo Aichun Biotechnology Co., Ltd
Protein Purifier
NegotiableUpdate on 02/02
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
The PPS-HD protein purifier is divided into three versions: PPS-10HD, designed specifically for purification tasks of scientific research users; PPS-100HD， Very suitable for optimizing resource utilization and improving productivity in large-scale preparation and purification; PPS-PilotHD， The system has a wide range of work flow and pressure, suitable for research and development of different scales, large-scale research, as well as small-scale and pilot scale production. PPS-HD supports multiple chromatography techniques and meets the automation requirements required to provide high purity.
Product Details
PPS-HD protein purifierOverview
PPS-HD is a modular protein purification system that facilitates reliable and stable purification. The system consists of a PPS-HD host and PPSN software. The system adopts a modular design, with all valves, detectors, and chromatography columns installed on the front side of the system, making it easy for operators to operate the PPS-HD instrument (Figure 1). Additional components such as valves, detectors, and sensors from various optional modules can be easily added to available locations. There are multiple guide rails on the front and side of the instrument for connecting the chromatography column bracket and equipment. The buffer tray at the top of the instrument provides a convenient storage area for containers and bottles. The instrument control panel displays the system status in real-time.
The lighter weight of the system makes it easier to place on laboratory workbenches, and the smaller system size makes it easy to install in standard refrigerators for purifying unstable samples. PPS-HD comes standard with two high-performance system pumps, a system pressure detector for column protection, a mixer, an injection valve, a UV detector, and a conductivity detector. PPS-HD has multiple optional modules that offer multiple possibilities. The design of the system flow path aims to minimize bandwidth effects, and the wetting materials used in the flow path are biocompatible and can tolerate commonly used solvents. The front of the instrument is designed with reserved module positions for installing optional valves and detectors to achieve flexible flow path configuration. Examples of PPS-HD system configuration flow paths are shown in Figures 2 and 3.
PPSN software allows for quick and easy creation of methods, control of operations, and analysis of results. PPSN software does not require professional programming skills, and the creation of tomographic methods is very simple.
The PPS-HD standard components and optional components will be detailed in the following chapters.
Figure 1 PPS-HD instrument host
Figure 2PPS-HD protein purification systemStandard configuration flow diagram
Figure 3PPS-HD protein purification system high configuration flow path diagram
system parameters
| control system | PPSN |
| Dimensions (width x height x depth) | 482 × 661 × 432 mm |
| Weight (excluding computer, sample pump, component collector) | The heaviest weight is 65 kg |
| power supply | 100–240 V, ~50–60 Hz |
| power consumption | 300 VA |
| Shell protection level | IP 21 |
System pump
| Pump type | Measurement type plunger pump |
| flow rate range | PPS-10HD: 0.001 to 10 mL/min PPS-100HD: 0.01 to 100 mL/min PPS-PilotHD: 1 to 400 mL/min |
| Flow rate specification | PPS-10HDAccuracy: ± 0.5% Precision: RSD<0.5% (conditions: 0.1 to 10 mL/min,<3 MPa, 0.8 to 2 cP) PPS-100HDAccuracy: ± 0.5% Precision: RSD<0.5% (conditions: 1.0 to 100 mL/min,<3 MPa, 0.8 to 2 cP) PPS-PilotHDAccuracy: ± 1% Precision: RSD<1% (conditions: 4 to 400 mL/min,<1 MPa, 0.8 to 2 cP) |
| pressure range | PPS-10HD: 0 to 20 MPa PPS-100HD: 0 to 10 MPa PPS-PilotHD: 0 to 2 MPa |
| Viscosity range | PPS-10HD: 0.35 to 10 cP PPS-100HD: 0.35 to 5 cP PPS-PilotHD: 0.35 to 5 cP |
Sample pump
| Pump type | Measurement type plunger pump |
| Dimensions (width x height x depth) | 215 × 210 × 370 mm |
| weight | 11 kg |
| flow rate range | 0.01 Up to 100 mL/min |
| Flow rate specification | Accuracy: ± 0.5% Precision: RSD<0.5% (conditions: 0.1 to 100 mL/min,<3 MPa, 0.8 to 3 cP) |
| pressure range | 0 Up to 10 MPa |
| Viscosity range | 0.35 Up to 5 cP |
mixer
| Mixing principle | Chamber with magnetic stirrer |
| Mixing tank capacity | PPS-10HD: 1 (standard) or 2 mL PPS-100HD 150: 2 (standard) or 5 mL PPS-PilotHD5 mL (standard), other volumes can be customized |
| Gradient flow velocity range | PPS-10HD: 0.1 to 10 mL/min PPS-100HD: 0.5 to 100 mL/min PPS-PilotHD: 2.0 to 400 mL/min |
| Gradient synthesis accuracy | PPS-10HD± 0.6% (conditions: 5% to 95% B, 0.6 to 10 mL/min, 0.2 to 2 MPa, 0.8 to 2 cP) PPS-100HD± 0.8% (conditions: 5% to 95% B, 2 to 100 mL/min, 0.2 to 2 MPa, 0.8 to 2 cP) PPS-PilotHD± 2% (conditions: 5% to 95% B, 4.0 to 400 mL/min, 0.2 to 1 MPa, 0.8 to 2 cP) |
valve
| type | Rotary valve |
| number of valves | Up to 6 |
| function | Standard configuration: Injection valve Optional: Inlet selector valve, backwash valve, chromatography column selector valve, outlet selector valve |
pressure sensor
| Location of sensors | Standard configuration: After the system pump Optional: after sample pump, before chromatography column, after chromatography column |
| scope | 0 Up to 20 MPa |
| accuracy | ±0.02 MPaOr ± 2%, whichever is greater |
Inlet valve
| entranceA | 12 or 6 entrances |
| entranceB | 12 or 6 entrances |
outlet valve
| export quantity | 6 |
| Volume of component collection | 0.01Up to 100000 mL |
| Delay volume (UV outlet valve) | 300 μL(Standard piping) |
UV detector
| wavelength range | DET-HD-L：280 nm DET-HD-MStandard configuration: 200~400 nm, optional 200~800 nm. Step size: 1 nm, simultaneous detection of three wavelengths | |
| liquidity pool | Standard configuration: Optical path length of 2 mm, pool volume of 5 μ l Optional: Optical path length of 5mm, pool volume of 8 μ l | |
| resolution | 0.01 mAU | |
| linear | ± 2% within the range of 0-2AU | |
| drift | 1x10-3AU/H 2 mmAt 280 nm in the flow cell | |
| noise | < 0.1 mAU | |
| Lamp working life | LED> 10000hour Deuterium lamp>2000 hours | |
Conductivity detector
| Reading range | 0.00mS/cmUp to 1000.00mS/cm |
| accuracy | ±0.01mS/cmOr ± 2%, whichever is greater, (within 0.3 to 300 mS/cm) |
| work pressure | 0Up to 5 MPa |
| Flow pool volume | Standard configuration: 24 μ L |
Temperature detector
| Reading range | 0°Cto99°C |
| precision | ±1°C， Range of 4 ° C and 45 ° Cinside |
pHdetector
| Reading range | 0.00 Until 14:00 |
| accuracy | ±0.1 pHUnit, within the pH range of 2 to 12 |
| work pressure | 0To 0.5 MPa |
| Flow pool volume | Standard configuration: 120 μ L |
Component Collector
| Number of components | Up to 100 |
| Collection tube type | 100(15 mL centrifuge tube) 25(50 mL centrifuge tube) 4A 250 mL triangular flask |
| Component volume | 0.1Up to 250 mL |
| Anti overflow mode | Clamp valve control |
| Dimensions (width x height x depth) | 370 × 305 × 425 mm |
| Delay volume | 500 μL(Standard piping) |
Bubble sensor
| Number of sensors | up to 3 |
| sensor position | Before the system pump Before the injection valve |
| Sensing principle | ultrasound |
Order Information
system
| Product Code | product |
| 99018224 | PPS-10HD L |
| 99018226 | PPS-10HD M |
| 99016665 | PPS-100HD L |
| 99016694 | PPS-100HD M |
| 99014325 | PPS-PilotHD L |
| 99014328 | PPS-PilotHD M |
System accessories and modules
pipeline
| Product Code | product |
| 99024238 | 1/16PTFE tube |
| 99021583 | 1/16 PEEKTube inner diameter 1mm |
| 99022095 | 1/16 PEEKTube inner diameter 0.25mm |
| 99021368 | 1/16 PEEKTube inner diameter 0.5mm |
| 99021253 | 1/16 PEEKTube inner diameter 0.75mm |
| 99022116 | 1/8PTFE tube |
| 99020392 | Pipe cutter (with blade) |
| 99020407 | Titanium alloy inlet filter |
| 99020408 | UHMWPEInlet filter |
joint
| productcode | product |
| 99031252 | 10-32Long plug |
| 99031253 | 10-32Short plug |
| 99031255 | 10-32Long hand twisted connector |
| 99031256 | 10-32Hand twisted connector |
| 99032707 | 5/16-24, 1/16 '' pipe joint |
| 99032706 | 5/16-24, 1/16 '' pipe clamp sleeve |
| 99032117 | 5/16-24, 1/8 '' pipe joint |
| 99032118 | 5/16-24, 1/8 '' pipe clamp sleeve |
| 99031249 | 5/16-24, 3/16 '' pipe joint |
| 99031248 | 5/16-24, 3/16 '' pipe clamp sleeve |
| 99035801 | M6 FTurn 10-32 M connector |
| 99031257 | 10-32 FConvert M6 M connector |
| 99032776 | 5/16-20 FConvert M6 M connector |
| 99030339 | 10-32through |
| 99031332 | Pump cleaning solution connector |
| 99031251 | 10-32 MSyringe connector |
+
Injection ring
| productcode | product |
| 99041398 | 100uLQuantitative ring |
| 99041399 | 500uLQuantitative ring |
| 99041401 | 1mLQuantitative ring |
| 99041402 | 2mLQuantitative ring |
| 99044053 | 5mLQuantitative ring |
| 99046124 | 10mLQuantitative ring |
pump
| productcode | product |
| 99052075 | PPS-10HDPlunger rod |
| 99052077 | PPS-10HDsealing ring |
| 99051213 | PPS-100HDPlunger rod |
| 99051204 | PPS-100HDsealing ring |
| 99051890 | 1 mLMixing pool |
| 99051891 | 2 mLMixing pool |
| 99051892 | 5 mLMixing pool |
| 99052135 | 0.2 MPaReverse pressure valve |
| 99051963 | 0.4 MPaReverse pressure valve |
| 99052123 | Bubble sensor |
detector
| Product Code | product |
| 99061110 | 2 mmliquidity pool |
| 99061111 | 5mmliquidity pool |
| 99062933 | Tungsten lamp |
| 99062934 | Deuterium lamp |
| 99062941 | pHelectrode |
valve
| Product Code | product |
| 99071480 | Two inlet solenoid valves |
| 99070902 | 6Position inlet valve |
| 99070905 | Injection valve |
| 99070906 | Recoil valve |
| 99070907 | Single column valve |
| 99070910 | Five column valve |
| 99070915 | outlet valve |
Technical specifications are subject to change without prior notice.
Our company reserves the right of final interpretation and modification.
Version: Third edition
Protein Purifier