Primary cells of human pancreatic epithelium

All products of our company are for scientific experiments only and are not intended for use outside of scientific experiments!

Product Name:Primary cells of human pancreatic epithelium
English name:Human Pancreatic Epithelial Cells
Specifications5×10⁵Cells/T25culture flask
Item No.GOY-01X0783
Classification:Primary human cells

Growth characteristics: Wall adhesion

Cell morphology:Epithelial like cells

Human pancreatic epithelial cells isolated from pancreatic tissue; The pancreas is divided into two parts: exocrine glands and endocrine glands. Exocrine glands are composed of acini and glandular ducts. The acini secrete pancreatic juice, while the glandular ducts serve as channels for the discharge of pancreatic juice. Pancreatic juice contains protease and lipase. Pancreatic juice is discharged into the duodenum through pancreatic ducts and has the function of digesting proteins, fats, and sugars. Endocrine glands are composed of cell clusters of different sizes called pancreatic islets, which are mainly composed of4Cell composition: alpha cells, beta cells, gamma cells, andPPCells. Alpha cells secrete glucagon, which increases blood sugar levels; β - cells secrete insulin, which lowers blood sugar levels; Gamma cell secretion inhibits the secretion of alpha and beta cells in a paracrine manner;PPCells secrete pancreatic peptides, which inhibit gastrointestinal motility, pancreatic juice secretion, and gallbladder contraction. Pancreatic stem cells are believed to be isolated from the endodermal pancreatic epithelium during development. In the subsequent process of pancreatic development, pancreatic stem cells differentiate into pancreatic islet cells (α, β, δ)PPCells, ductal epithelial cells, and acinar cells. There are also a large number of mesenchymal derived cells in pancreatic tissue, including fibroblasts, endothelial cells, smooth muscle cells, and astrocytes, with fibroblasts accounting for the majority. To isolate and culture pancreatic epithelial cells in vitro, mesenchymal derived cells, especially fibroblasts, must be excluded. The commonly used methods for removing fibroblasts include mechanical scraping, protease digestion, and collagenase digestion. The pathological changes of pancreatic epithelial cells are of great significance in the occurrence of acute and chronic pancreatitis.

Method Introduction:

The human pancreatic epithelial cells isolated in the company's laboratory were prepared by collagenase digestion and selected through epithelial cell specific culture medium, with a total cell count of approximately5×10?cells/Bottle.

Quality inspection:

Human pancreatic epithelial cells isolated from the company laboratoryCytokeratin-19Immunofluorescence identification, purity can reach90%Above, and not containingHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, fungi, etc.

1. Preparation of culture medium and culture cryopreservation conditions:

Prepare DMEM high sugar, 10% high-quality fetal bovine serum.

1) Cultivation conditions: Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%.

2) Cryogenic solution: 90% culture medium, 10% DMSO, ready to use and prepared. Liquid nitrogen storage.

2. Cell processing:

1) Resuscitate cells: Quickly shake and thaw a cryovial containing 1mL of cell suspension in a 37 ℃ water bath, then add 4mL of culture medium and mix well. Centrifuge at 1000RPM for 4 minutes, discard the supernatant, add 1-2mL of culture medium, and blow evenly. Then add all cell suspensions to the culture bottle and culture overnight (or add the cell suspensions to a 10cm dish, add about 8ml of culture medium, and culture overnight). The next day, change the solution and check the cell density.

2) Cell passage: If the cell density reaches 80% -90%, passage culture can be carried out.

3) Cell cryopreservation: When the cells are in good growth condition, cell cryopreservation can be performed. When freezing adherent cells, discard the culture medium and add a small amount. After the cells become round and fall off, add about 1ml of serum containing culture medium to the freezing tube, and then add 10% DMSO for freezing.

1. After receiving the cells, first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please contact us in a timely manner.

2. Carefully read the cell manual to understand cell related information, such as cell morphology, culture medium used, serum ratio, required cytokines, etc.

3. Wipe the surface of the cell vial with 75% alcohol and observe the cell state under a microscope. Due to transportation issues, a small amount of adherent cells may detach from the bottle wall. The cells should be placed in a culture box and incubated overnight before being taken out for observation the next day. At this point, most cells will adhere to the wall. If the cells still cannot adhere to the wall, please use trypan blue staining to determine cell viability. If it is confirmed that the cell viability is normal, please centrifuge the cells and culture them again with fresh culture medium; If the staining result shows that the cells are inactive, please take a photo and contact us in a timely manner. After confirming the information, we will send it to you again for free.

4. Please ask the customer to use the same culture medium for cell culture under the same conditions. Excess culture medium in the culture bottle can be collected for future use. When cells are passaged, they can be mixed in a certain proportion with the culture medium provided by the customer to gradually adapt to the culture conditions; Suggest purchasing the provided culture medium directly.

5. It is recommended that customers take several photos of the cells in the first 3 days after receiving them, recording the cell status for communication and exchange with the technical department of the Microbial Strain Query Network.

6. This cell can only be used for scientific research and cannot be used for clinical applications.

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