Preparation of liquid chromatography equipment manufacturer

Preparation of liquid chromatography equipment manufacturerThe HT7200A preparative liquid chromatography developed by Suzhou Huitong is a modular preparative chromatography system with high functionality, compatibility, and efficiency. Compact structural arrangement for preparing separation and purification equipment with high flux and efficient purification speed. Adopting dual wavelength simultaneous detection, precise flow rate, and supporting multiple component fractionation collection methods; Balancing the connection between medium pressure glass columns and high-pressure stainless steel columns. Widely used in the purification and preparation of active ingredients such as natural products, traditional Chinese medicine, organic synthetic compounds, and protein biomolecules.

Preparation of liquid chromatography instrumentTechnical parameters:

• Pump head material	316L stainless steel, titanium alloy material, PEEK material pump head optional
  Flow velocity range	0.01-200ml/min gradient
  Traffic readiness	±0.5%
  pressure range	0-30Mpa
  pressure pulsation	≤0.2MPa
  Pressure accuracy	≤0.1%
  wavelength range	190-700nm
  Light source	Deuterium lamp+tungsten lamp
  wavelength accuracy	±2nm
  Flow pool path length	Half prepared 2mm, prepared 1mm
  Flow pool volume	Half prepared 5ul, prepared 10ul
  collector	Fraction collector
  collection system	X-Y robotic arm
  collection mode	Manual mode, program mode, spectrum mode, external control mode
  switching valve	PEEK three-way solenoid valve
  Collecting containers	10 * 10 test tubes (15mm, 18mm, 25mm), centrifuge tube, 96 well plate, 3 * 3 500ml solvent bottle, beaker
  communication protocol	Digital interface (RS232 \ \ RS485), analog signal interface, 3.5 headphone jack
  software system	Huitong Chromatography independently developed software to control the program settings and operation of instruments such as pumps, detectors, collectors, etc
  control interface	PC software workstation compatible with Windows 7/Windows 8/Windows 10, independent instrument interface control, USB interface+RS232/RS485 interface, etc.
  Sample injection device	Manual preparation of injection valve, quantitative ring optional
  Sample loading method	Pump sampling, solid and liquid sampling

Characteristics of preparing liquid chromatography:
1. Adopting a dual plunger pump head design, the infusion pulsation is lower;
2. The pump head has a flushing function to prevent wear and tear from inorganic salts, ensuring the service life of the plunger rod and sealing ring;
3. Pressure setting alarm device, pressure measurement and overpressure protection system;
4. It has solvent compressibility compensation, fully automatic real-time flow compensation, and manual setting compensation;
5. Adopting a full touch screen display with *, it is more convenient to independently write programs for system operation;

Preparation of liquid chromatography instrumentThe usage steps
1、 Power on
1. Connect the computer and instrument power (before connecting the power, you can first take the suction head into the mobile phase bottle to be used);
2. Turn on the power switch of the instrument, usually from top to bottom, with the detector switch at the bottom;
3. Check if the mobile phase is sufficient and if the solvent amount recorded on the instrument is correct;
4. Turn on the computer;
5. Open the instrument online software to enter the interface, click start to set the pump flow rate to 10, and there should be liquid flowing out of the instrument at this time;
6. Click on balance and then click on each wavelength and adjust;
After a period of time, the sample can be injected once the pressure stabilizes.
2、 Sample injection
1. Twist the sampler to load up and extract a certain amount of the liquid to be separated,
Quick injection, what was done was to aspirate 0.2 milliliters;
2. Click the "Run Control" button in the menu bar of the control interface to set the sample information storage path (to be done every time);
3. Twist the sampler to enter the unload state and start timing the retention time;
4. Peak connection;
5. After all peaks have appeared, click on the interrupt button in the menu bar to prepare for repeated injection.
3、 Shut down
1. After clicking the interrupt button, rinse for a few minutes and set the flow rate to 0. After the pressure reaches 0, transfer the suction head to methanol and set the flow rate to 10;
2. It usually takes half an hour to rush until there is nothing left to rush down. At this point, the pressure is usually already stable;
3. Set the flow rate to 0 and click 'Close' on the interface (next to 'Start');
4. Close the window, and click 'OK' on all pop-up windows, such as' OK '.