Pre processing method for sugar detection in meat products - purification using sugar specific SPE column

The meat products in this article, such as vegetarian burger, marinated tender chicken breast, original fried chicken, spicy fried chicken, basic flavored chicken shreds, spicy chicken cutlets, chicken wing paste, chicken hot dogs, vanilla chicken leg cutlets, etc., are seasoned with complex seasonings during the processing, which poses great difficulties for quantitative analysis of sugar in meat products.

There are two difficulties in quantitative analysis of sugar in meat products:

1、 There is no ready-made quantitative analysis standard method to refer to.

2、 Clean the sample solution that does not have a bottom on the machineLC/ELSDAfterwards, it is highly prone to damage and blockage of sugar specific chromatography columnsELSD atomizer consumes significant maintenance costs;

To solve this problem, Juyan Technology has developedFaAEx-AD2 sugar specific SPE purification column, exploring suitable pretreatment operation steps and instrument analysis conditions.

After this analysis method has been validated, industry peers will be invited to conduct practical verification, and feedback from peers will be providedThe purification effect of FaAEx-AD2 sugar specific SPE column is good!

This article will introduce two methods for detecting sugar in meat products:

Method 1: Use Giant ResearchFaAEx-AD2 sugar specific SPE column purification, in order toLC/ELSDLegal analysis;

Method 2: ReferenceGB/T 9695.31-2008 method, using spectrophotometry and direct titration for analysis.

Method 1: Use Giant ResearchFaAEx-AD2 sugar specific SPE column purification

Taking Thai flavored leg meat slices as an example, test fructose（Fructose, Shan pear alcohol（Sorbitol）Galactose（Galactose）Glucose（Glucose）Sugar cane（Sucrose）Lactose（Lactose）And maltose（Maltose）Add the sampleTake the supernatant from 10ml of 50% ethanol solution, sonicate, shake, dilute, and centrifuge.

Pass the supernatant throughFaAEx-AD2 sugar specific column for impurity removal (only one step is needed to pass through the column), obtain the target solution, filter it, and then load it onto the machineLC/ELSDPerform quantitative analysis.

（1) Pre processing steps:

Sample homogenization

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取Add 1g of sample to a centrifuge tube and 10ml of 50% ethanol solution

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ultrasonic vibration20min

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High-speed oscillation10min

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withDilute 50% ethanol solution to 20 mL (solid sample: add 10mL directly)

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Centrifuge at 6000rpm for 30 minutes

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取10ml of supernatant was passed through a FaAEx-AD2 sugar specific SPE column, and the filtrate was analyzed on the machine

(No need to activate equilibrium, one-step method to obtain the target substance)

（2) The spectrum analyzed by LC-MS is as follows:

（3) Summary:

useFaAEx-AD2 sugar specific SPE column purificationAfterwards, there are two benefits to analyzing on the computer:

1Fructose（Fructose, Shan pear alcohol（Sorbitol）Galactose（Galactose）Glucose（Glucose）Sugar cane（Sucrose）Lactose（Lactose）And maltose（Maltose）ThisThe recovery rate of 7 types of sugars is between 80-120%.

2Effectively extend the service life of sugar specific chromatography columns and avoid blockageELSD atomizer greatly reduces the cost of consumables and accessories.

3Why is there still a need to develop purification operations such as extraction, ultrasound, and centrifugation that have already been carried out in pre-processingFaAEx-AD2 sugar specific column for impurity removal again?

Answer: Because the supernatant obtained by centrifugation is filtered through a filter membrane and analyzed on the machine, after several injections of the sample, the sugar specific chromatography column is contaminatedThe ELSD atomizer also quickly became clogged, making it difficult to perform daily quality control management.

The supernatant obtained by centrifugation is subjected toAfter purification with the FaAEx-AD2 sugar specific column, this problem has been effectively solved. Users who need sugar testing in meat products on a daily basis should continue to repurchase the FaAEx-AD2 sugar specific column.

Method 2: ReferenceGB/T 9695.31-2008 standard method

Regarding the detection of total sugar in meat products,weThere is a standard method, which is:《GB/T 9695.31-2008 Determination of Total Sugar Content in Meat Products,Using glucose as a control substance.

The first method is applicable to the determination of total sugar content in meat products，Using spectrophotometry;

The total sugar content in the sample is calculated as glucose.

The second method is applicable to the determination of total sugar content in meat products without starch, usingDirect titration method for measuring sugar

Summary:

1Detection of sugar in meat products, if usedGB/T 9695.31-2008 standard method, the pre-processing steps are complex, time-consuming, and require a lot of manpower. The sensitivity ratio of the analysis results isLC/ELSDThe method is low and almost impossible to conduct quantitative analysis.

2adoptLC/ELSDThe difficulty in analyzing the sugar content in meat products lies in how to effectively remove impurities from complex samples. passAdd to the sampleThe recovery rate of 10ml of 50% ethanol solution, ultrasound, shaking, constant volume, centrifugation, and filtration steps is acceptable, but the impurity removal effect is poor. After several injections, the sugar specific chromatography column is contaminated, and even the ELSD atomizer is blocked, increasing the consumption cost of consumables and instrument accessories.

3Centrifuge the supernatant and pass it through a one-step methodThe purification and filtration of FaAEx-AD2 sugar specific SPE column can effectively remove impurities, prolong the service life of sugar specific chromatography column and ELSD, and the HPLC baseline is more stable with better recovery performance (80-120%).