Porcine primary bone marrow mesenchymal stem cells

NegotiableUpdate on 05/06

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Overview

Porcine primary bone marrow mesenchymal stem cells are bone marrow stromal stem cells that not only provide mechanical support to hematopoietic stem cells (HSCs) in the bone marrow, but also secrete various growth factors (such as IL-6, IL-11, LIF, M-CSF, and SCF) to support hematopoiesis.

Product Details

　　Porcine primary bone marrow mesenchymal stem cellsDetailed description:

Bone marrow mesenchymal stem cells are bone marrow stromal stem cells that not only provide mechanical support to hematopoietic stem cells (HSCs) in the bone marrow, but also secrete various growth factors (such as IL-6, IL-11, LIF, M-CSF, SCF, etc.) to support hematopoiesis. Bone mesenchymal stem cells (BMSCs) have the potential for multi-directional differentiation and can promote the regeneration of mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, fat, and matrix.

In the bone marrow, BMSCs account for 0.001% to 0.1% of the total number of nucleated cells, and their content is extremely low. However, due to the large number of seed cells required for tissue engineering, the technical difficulty of isolating BMSCs from rodent bone marrow has limited the progress of many experiments. The isolation and cultivation of BMSCs with high purity, strong vitality, and uniform biological characteristics in vitro is crucial for tissue engineering and in vivo and in vitro experiments of cells.

Bone Marrow Mesenchymal Stem Cells (BMSCs) are a type of stem cell with multipotent differentiation potential isolated from pig bone marrow. They have important application value in tissue engineering and regenerative medicine because they can differentiate into multiple cell types, including osteoblasts, chondrocytes, adipocytes, muscle cells, etc.

In the study, pig BMSCs were used for various applications, including:

1. Tissue engineering: used for repairing and regenerating damaged tissues and organs.

2. Cell therapy: as a potential means of treating certain diseases, such as improving pancreatic islet cell dysfunction through transplantation.

3. Disease model: used as a model cell in studying disease mechanisms and drug screening.

In addition, the cultivation and expansion of pig BMSCs require specific culture conditions, including suitable media, growth factors, and cytokines. During the cultivation process, cells need to be regularly passaged and frozen to maintain their biological characteristics and functions.

　　Porcine primary bone marrow mesenchymal stem cellsCharacteristics:

1) The organization is derived from normal bone marrow blood tissue of experimental animals.

2) Cell identification: CD44 immunofluorescence staining is positive.

3) After identification, the purity of the cells was found to be higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: spindle shaped cells, irregular cells, adherent culture.

Transportation mode:

Low temperature: (1) Transport in 1mL cryovials packaged with dry ice. After receiving, store in a -80 degree freezer overnight and transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the cryovial cap has fallen off, is damaged, or the cells are contaminated, please contact us immediately.

At room temperature: (2) T25 bottles of revived surviving cells will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.

Bio-Safety:

1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal.

2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells. Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.