Porcine interleukin-10 (IL-10) detection kit

Linear range:0.78pg/mL-50 pg/mL

1. intended use

This kit is used for detecting serum, plasma, tissue and other samplesPorcine interleukin10(IL-10)Concentration,For research purposes only.

2. Detection Principle

The reagent kit adopts the principle of double antibody sandwich method: the capture antibody is wrapped on an enzyme-linked immunosorbent assay (ELISA) plate, the analyte in the sample and calibration sample is captured, and horseradish peroxidase labeled enzyme labeled detection antibody is added, which combines to formAntibody-antigen-antibody-HRP”Immune complex, addedTMBAfter color development, if the analyte in the sample appears blue, add a stop solution to stop the reaction. During the detection process, all free components were washed away and analyzed using an enzyme-linked immunosorbent assay (ELISA) reader450 nmTest locationODThe value, color depth, and the content of the analyte in the sample are directly proportional. The concentration of the analyte in the sample can be calculated by drawing a standard curve.

3. includedExperimental materials

If separate procurement is requiredUniversalPlease provide the corresponding componentscomponentname

4. Required but not included experimental materials

·Distilled water or deionized water, disposable centrifuge tubes, disposable gloves and other consumables;

·Pipettes, multi-channel pipettes, and matching gun heads;

·Beakers, measuring cylinders, reagent bottles and other containers;

·Sealing film or other alternative materials used for sealing microporous plates;

·Microplate oscillator (if needed), centrifuge, vortex oscillator and other auxiliary equipment;

Constant temperature incubator, constant temperature water bath, enzyme-linked immunosorbent assay reader, washing machine.